GCaMP6 can reliably report activity during in vivo conditions from hundreds of neurons with excellent signal-to-noise ratio
Neural activity causes rapid changes in intracellular Ca2+. Calcium imaging experiments have relied on this principle to track the activity of neuronal populations and to probe excitation of small neurons and neuronal micro compartments. Genetically encoded protein sensors can be targeted to specific cell types for non-invasive imaging of identified neurons and neuronal compartments over chronic timescales. The new family of ultrasensitive protein calcium sensors (GCaMP6 or higher versions) outperforms other sensors in cultured neurons and in zebra fish, flies and mice in vivo.
We have developed new hardware and software tools to record GCaMP6 activity during in vivo conditions from up to several hundred neurons with excellent signal-to-noise ratio. Our method allows real-time visualization of the response of the neuronal assemblies. Simultaneous analysis of multiple cell activity with the simultaneously recorded electrophysiological data is also possible.
In vivo recording of GCaMP6 activity from 120 neurons as seen in the MES software. Each column corresponds to the Ca2+ resposne of a given preselected neuron. Examplified transients from the color coded regions are shown on bottom left.
Spontaneous calcium transients of selected GCaMP6 labeled neurons and dendrites in response to visual stimuli (moving gratings).