The fastest imaging of 3D dendritic arborization by Femto3D AcoustoOptic
Dendritic and spine activity during small motions
3D ribbon scanning
An extension of the 3D multiple-line scanning performed by Anti-motion technology is 3D ribbon scanning, which makes it possible to image ribbon shaped surfaces containing dendrites and the neighboring areas. Figures show 3D ribbons encompassing seven dendritic segments with their spines of a GCaMP6-labeled layer II/III pyramidal neuron measured within the brain of a living mouse. The seven ribbons were projected into a 2D image ordering dendrites above each other for better visualization, and activity was recorded from 40 selected spines and visualized in the form of classical Ca2+ transients.
Dendritic and spine activity during large motions
3D snake scanning
3D snake scanning is a volume extension of ribbon scanning and contains the entire 3D environment of the dendrite. It therefore supports imaging of dendrites in larger animals, or behavioral protocols, where the amplitude of motion can be large. Figure shows fast snake scanning performed at 10 Hz in the selected dendritic region of a V1 pyramidal neuron.
Imaging along the entire length of dendritic arbor
3D multi-layer scanning
Imaging multiple frames with different sizes at any position in the scanning volume can be used to follow all events propagating along the cell. The lower figure shows imaging of the entire length of a pyramidal neuron in vivo, where the small scanned rectangles cover the apical dendrite across multiple layers. Motion compensation enables us to record fluorescent signals and responses to visual stimuli while the animal is running on a treadmill. See also Szalay et al., Neuron, 2016.