Full-field illumination

FemtoSmart Resonant equipped with LED light source

The entire FOV can be stimulated with the LED source above the objective. LEDs are available at different wavelengths, exciting ChR2 at 473 nm or NpHR at 561 nm. The light source provides homogeneous illumination, and the light impulses are precisely timed and highly repeatable. The FemtoSmart Resonant microscope follows the changes over the whole field of view at a resolution of 31 frames per second.

Stimulation along ROIs

FemtoSmart Galvo equipped with Multiple beam path extension

To stimulate cells or subcellular components selectively, the best solution is using the FemtoSmart Galvo equipped with a secondary laser to steer the laser beam rapidly through optimized scanning patterns such as such as point, line, spiral, zigzag. While scanning along points and lines, which allow stimulation on in precise locations on spines or dendrites, the spiral and zigzag patterns covering larger region enable more molecules to be stimulated symultaneosly on the soma resulting activation. We offer a continuous laser tuned to 473 or 561 nm for ChR2 or NpHR activation, respectively. Precise two-photon activation of these molecules is also a viable option.

Simultaneous photoactivation and imaging

FemtoSmart Dual equipped with Multiple beam path extension

The FemtoSmart Dual microscope contains a galvo and a resonant scanner which function in tandem to combine the advantages of both the galvo and resonant microscopes. This is the best solution for simultaneous photostimulation and high-speed imaging.

Stimulation along ROIs distributed in 3D

Femto2D Galvo with Holographic stimulation unit

The holographic stimulation is an extremely flexible method which produces simultaneous illumination in variable shape and size of multiple regions in the three-dimensional sample. The simultaneous illumination of different regions is established by diffractive Spatial Light Modulator (SLM). The SLM consists of a parallel-aligned nematic liquid crystal layer which in the crystals disperse and shape the incoming laser beam and change the phase of light. This converted holographic beam excites multiple spots with arbitrary shapes in pre-determined depths simultaneously. The holographic unit is controlled by our control software, which calculates the corresponding phase-hologram and addresses the pattern to the crystal layer.