• Piezo objective positioner kit

    for fast Z-stack and 3D imaging

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Technology Overview

A Piezo objective positioner kit enables the microscope to change the focal point by mechanically moving the objective. With this module the microscope is able to collect signals from different depths fast enough to resolve biological activity in 3D samples. We can equip most of our microscopes with two types of piezo: piezo specialized for large scanning volumes moves the objective in a 400 µm travel range with up to 30 Hz speed, and the faster one moves it in 100 µm volume with up to 100 Hz (the refresh rate depends on the scanning volume and the size of the objective).

Features

  • positions objectives in the Z direction with nm resolution

  • travelling range 100 or 400 µm (depends of the piezo’s type)

  • up to 100 Hz frequency in resonant mode

  • millisecond step-and-settle

  • any types of objectives can be attached

  • no influence on the optical path

Benefits

  • Femtonics microscopes containing galvo scanner equipped with piezo objective positioner and RollerCoaster software module support 3D trajectory scanning

  • 3D trajectory scanning allows collecting signals from dendrites arbored in the 3D tissue and to resolve biological activity thanks to the high scanning speed

  • Femtonics microscopes containing resonant or galvo scanners are able to image a 3D volume scanning

  • 3D volume scanning enables you to reveal activity of the neural network or other cell groups

  • fine positioning along the tilted axis when using tilting objective module

Optional modules

Femtonics Technology