Neural network imaging
The Femto3D Atlas enables the spatial and temporal complexity of neuronal coding to be resolved by scanning distributed points as regions of interest (ROIs) in a large 3D volume. The high speed of random-access point scanning (30 kHz/ROI) facilitates to measure several thousand cells near-simultaneously by restricting the imaging to sub-regions of the 3D volume. The Cell3DFinder software module finds cell centers in 3D image stacks automatically and can display them as a set of points. Ratio imaging software module allows real-time ratio metric analysis by calculating relative fluorescence, and contains also batch analysis tools. The figure (A-D) shows the changes in Ca2+ concentration of 2000 cells from the visual cortex of a GCaMP-expressing mouse as a function of time. See more Wertz et al., Science, 2015; Katona et al., Nat Meth, 2012.