Multiple line scanning has been developed for researchers who want to resolve dendritic and even spine activity of neurons approaching near real-time measurement mode. During this scanning mode, the X and Y mirrors of the galvo scanner direct the laser beam flexible along straight lines or complex curves. The scanner spends most of its time collecting signals from these lines, while the intermediate sections between the lines are skipped. In this way, the scanning speed and the signal-to-noise ratio (SNR) of the signals sampled from the multi-site ROIs increases 3- to 4-fold compared to frame scanning.
Using the two scanners function in tandem is a perfect solution for photostimulation
The galvanometric scanner directs the laser to cells or subcellular components selectively. The selectivity is established by scanning along arbitrary line patterns placed on cell bodies or dendritic segments. With the resonant scanner, you can follow the changes in the cells of a neural network collecting imaging data simultaneously by high-speed frame scanning of the surrounding area. Timing of photostimulation and gating of the detectors are controlled by protocols designed on user-friendly GUI.