Deep brain imaging

Thanks to two-photon laser technology and our optical developments, you can study cell bodies and dendrites at a high spatial resolution down to a depth of 850 µm with no photodamage. The 3D slicer module of the control software implements XZ or YZ sectioning and projection of the Z-stacks and enables 2D visualization of 3D stacks projected to any of the three axes.

Calcium imaging

The fast scanning speed on user-defined, separated regions ensures precise and repeatable measurements of rapid changes in the Ca2+-level of neurons and their dendrites. The ratio-imaging software tool offers algorithms for eliminating background noise, and determinates the relative fluorescence changes, displaying them as transient curves as a function of time. The batch analysis tool contains efficient tools for analyzing entire multi-ROI measurement

Intravital imaging

The infrared excitation laser can penetrate thick specimens, enabling living cell behavior in intact tissues and organs to be visualized at high resolution for extended periods with no phototoxicity: essential for morphological studies. The metaprotocol module of the control software automates these measurements on a timescale of seconds by letting you run freely composed sequences of image acquisition.

Parallel electrophysiology

Hardware and software tools help parallel two-photon imaging and aligned electrophysiological recordings. Precise triggering the external recording ensures time aligned measurement of the electrophysiology and imaging, providing different aspects for studying the neuronal cell and network activity. Software module helps automatic importing recorded traces for parallel analysis. Figure shows calcium imaging and patch-clamp recording in an OGB-1 and Alexa-594 filled hippocampal neuron.

Use the unique scanning patterns for uncaging and optogenetics studies. FemtoSmart: ideal for photostimulation.