Advanced 2D scanning methods
The advantages of the galvo scanner combined with the intelligent, user-friendly control software enables the user to use many scanning patterns covering the ROIs distributed across the field of view. These patterns have been developed based on the most frequent requests by neuroscientists. For example, multiple frame scanning focuses on cell bodies, multiple line scanning enables us to follow action potentials along dendrites, and random-access point scanning allows measurement or photo stimulation of subcellular components of the highest temporal resolution. Many features of the software, such as real-time display, analysis functions, ∆F/F calculations and integrated parallel data acquisition of electrical recordings promote greater understanding of the physiological processes under the focus of your research.
High signal-to-noise ratio
Scanning only the relevant part of the field-of-view, and skipping the background, result in a very high signal-to-noise ratio.
Photon collection efficiency is enhanced thanks to the shortest possible optical path realized by our patented travelling detector system
The most sensitive GaAsP photomultipliers (quantum efficiency less than 40%) collect scattered photons.
Folded frame scanning
This patented method enables imaging of a confined area along a line, where the shape of the selected regions can be straight or curved. This advanced scanning method is useful for imaging single cell bodies in different regions of the specimen, or following events along winding dendrites with their protrusions, even while the tissue is moving.
Uncaging, optogenetics, and other photo stimulation techniques are also supported by our unique scanning patterns and their combinations. Random-access point scanning can be used for stimulation in femtoliter volumes near dendritic spines where the duration of the stimulation, can be set from microseconds to seconds precisely to the experiment. The evoked signals can be followed along the dendrite by line scanning near simultaneously with the photo stimulation. The microsecond-scale switching time between the stimulation and imaging is achieved by using of a Pockels cell and gated detectors.