OVERVIEW

The DNI-Glutamate is a biochemically masked, dinitro-indoline derivative of the neurotransmitter glutamate. It has been engineered for a high quantum yield, it releases Glu more rapidly by the effect of two-photon irradiation, than other caging scaffolds.

  • name: 4-methoxy-5,7-dinitroindolinyl-L-glutamate trifluoroacetate
  • molecular formula: C16H17N4O10F3
  • mw: 482.32
  • standard packaging size: 14.3 mg
The caged compound exists as trifluoroacetic acid salted form (DNI-Glu*TFA), the TFA salt of compound ensures good solubility, stability and low hygroscopicity of the complex.

ADVANTAGES

  • seven-fold higher quantum yield than MNI-Glu
  • high excitatory postsynaptic potential (EPSP) and high calcium transient as a response of the photorelease
  • lighter illumination sufficient to elicit the same response as with alternative compounds
  • less photodamage
  • stable and soluble
We especially recommend
  • eliciting large transients or regenerative activity
  • multiple illumination of the same structure
  • receptor mapping experiments
  • primarily used for in vitro experiments
  • photostimulation at 740 nm with femtosecond laser

Figure: Photoactivation of DNI-Glu close to a dendritic segment of a hippocampal interneuron. The elicited calcium responses were measured by line scannings along the dendrite. The stimulation and the imaging was performed by Femto2D-Galvo.

REFERENCES