OVERVIEW

The chamber is used for live-cell imaging. It allows increased oxygenating of acute slice preparations up to 800 µm thickness maintaining cells in a healthy state. Whole-cell patch-clamp and field recordings can be performed simultaneously allowing the recording of spontaneous network oscillations.

DUAL PERFUSION CHAMBER KIT

We offer dual perfusion chamber in a complex kit, which contains:
  • unique dual perfusion policarbonate chamber
  • sliceholder
  • bubbletrap
  • dual-channel in-line solution heater
  • temperature controller
  • peristaltic pump

BENEFITS

In contrast with the single perfusion, dual-superfusion of submerged slices increases the vitality of cells, since e.g. oxygen as well as other materials can freely and effectively diffuse with high flow rate from both sides into the thick slices. Therefore physiological network oscillations such as sharp waves ripples (SPWs) are more preserved, furthermore, increased network activity may generate dendritic regenerative activities.

Figure shows enhanced action potential backpropagation during hippocampal SPW activity measured in dual-perfusion chamber (Chiovini et al 2010, Neurochemical Research).

REFERENCES

Enhanced Dendritic Action Potential Backpropagation in Parvalbumin-positive Basket Cells During Sharp Wave Activity. B. Chiovini, GF. Turi, G. Katona, A. Kaszas, F. Erdelyi, G. Szabo, H. Monyer, A. Csakanyi, ES. Vizi, B. Rozsa Neurochemical Research (2010)


Maintaining network activity in submerged hippocampal slices: importance of oxygen supply. Hajos N, Ellender TJ, Zemankovics R, Mann EO, Exley R, Cragg SJ, Freund TF, Paulsen O,Neurochemical Eur J Neurosc (2009)


Establishing a physiological environment for visualized in vitro brain slice recordings by increasing oxygen supply and modifying aCSF content. Hajos N, Mody I, Neurosci Methods (2009)


Roller Coaster Scanning reveals spontaneous triggering of dendritic spikes in CA1 interneurons. G. Katona, A. Kaszas, G. F. Turi, N. Hajos, G. Tamas, E.S. Vizi, B. Rozsa, PNAS (2011)


Cellular and network mechanisms of rhythmic recurrent activity in neocortex. Sanchez-Vives MV, McCormick DA, Nat Neurosci (2000)