OVERVIEW

The Femto2D-Dual microscope gives the ability to perform dual scanning using both galvo and resonant scanners in tandem. With galvo scanner you can zoom to tiny structures such as dendritic spines and jump quickly between these zoomed regions. In contrast, with resonant scanner you can capture images with high frame rate to follow rapid changes on the FOV.

COMBINED ADVANTAGES OF GALVO AND RESONANT SCANNERS

Choose the best scanning mode which fits your imaging requirements:
  • resonant scanner ensures high speed image acquisition up to 31 frames per second (fps) over the whole FOV
  • galvo scanner provides flexible ROI scanning opportunities.

HIGH SIGNAL-TO-NOISE RATIO

Revealing subtle changes of the evoked signals based on the following achievements:
  • the shortest possible optical path allows enhanced photon collection efficiency thanks to our patented travelling detector system,
  • using the highest sensitivity GaAsP photomultipliers (quantum efficiency >40%) optimized for scattered photons,
  • carefully chosen high quality filtersets.

MODULARITY

The modular nature of the microscope supports to extend it with many upgrade elements ensuring a future proof performance in all kinds of optical-physiology measurements (dendritic imaging, uncaging, optogenetics, parallel electrophysiology, etc.).

FULL SPECIFICATION

REFERENCES

MULTIPLE LINE SCANNING

Our patented multiple line scanning mode allows unique enhancement of the signal-to-noise ratio in multisite measurements. The scanner spends the most of time by collecting signals from the ROIs while the intermediate sections are skipped within 60-100 µs. A typical 3-4 fold improvement of the SNR can be achieved compared to frame scanning.

FOLDED FRAME SCANNING

This approach allows imaging of a confined area along a line. The shape of the selected regions can be straight or curved while the position is arbitrary. This advanced scanning method is useful for imaging just cell bodies in the different places of the specimen or following events along winding dendrites with their protrusions.

TIME-LAPSE IMAGING

While two-photon excitation ensures depth penetration and fine spatial resolution, the high frame scanning rate of the resonant scanner ensures temporal resolution allowing observation of rapid events in living cells, neuronal networks or other circuits.

3D VOLUME SCANNING

Two new upgrade modules support near real-time, XYZ-T imaging of 3D volumes:

Both solutions combined with the fast frame scanning feature of resonant scanner allow fast, near real time scanning of a selected 3D volume.

DEEP BRAIN IMAGING

Thanks to the two-photon laser technology and our optical developments, you can study nervus system with distinguishable cell bodies and dendrites down to 850 µm depth in the brain of behaving animals without photodamage.

CALCIUM IMAGING

To measure changes in Ca2+-levels is a powerful means for monitoring the activity of neural circuits. Calcium-indicators such as OGB-1 fluorescent dye or GCaMP fluorescent molecule can respond to the binding of Ca2+-ions by changing their fluorescence properties. To follow changes, the two kinds of scanners give the following possibilities:
  • resonant scanner allows to perform rapid series of measurements revealing events on a large field,
  • and using galvo scanner, you can measure the Ca2+-level on small, predetermined regions with high signal-to-noise ratio.

PARALLEL ELECTROPHYSIOLOGY

Hardware and software tools help parallel two-photon imaging and electrophysiological recordings, which provides different aspects to study the neuronal cell and network activity.

INTEGRATION

MESc fully integrates the control of all hardware units in the microscope such as core components (scanner, PMTs), light path actuators, various sample stages, auxiliary digital and analog channels.

ROI SELECTION

Numerous software tools support many kinds of 2D scanning pattern generation with high flexibility: point, line, curved line and areas with various shape can be selected thereby improving the efficiency of imaging.

REAL-TIME DISPLAY
AND ANALYSIS

Integrated, quantitative intensity-based calculations allow following real-time fluorescence changes and simultaneous analysis.

OVERVIEW

OVERVIEW

The Femto2D-Dual microscope gives the ability to perform dual scanning using both galvo and resonant scanners in tandem. With galvo scanner you can zoom to tiny structures such as dendritic spines and jump quickly between these zoomed regions. In contrast, with resonant scanner you can capture images with high frame rate to follow rapid changes on the FOV.

COMBINED ADVANTAGES OF GALVO AND RESONANT SCANNERS

Choose the best scanning mode which fits your imaging requirements:
  • resonant scanner ensures high speed image acquisition up to 31 frames per second (fps) over the whole FOV
  • galvo scanner provides flexible ROI scanning opportunities.

HIGH SIGNAL-TO-NOISE RATIO

Revealing subtle changes of the evoked signals based on the following achievements:
  • the shortest possible optical path allows enhanced photon collection efficiency thanks to our patented travelling detector system,
  • using the highest sensitivity GaAsP photomultipliers (quantum efficiency >40%) optimized for scattered photons,
  • carefully chosen high quality filtersets.

MODULARITY

The modular nature of the microscope supports to extend it with many upgrade elements ensuring a future proof performance in all kinds of optical-physiology measurements (dendritic imaging, uncaging, optogenetics, parallel electrophysiology, etc.).

FULL SPECIFICATION

REFERENCES

TECHNOLOGY

MULTIPLE LINE SCANNING

Our patented multiple line scanning mode allows unique enhancement of the signal-to-noise ratio in multisite measurements. The scanner spends the most of time by collecting signals from the ROIs while the intermediate sections are skipped within 60-100 µs. A typical 3-4 fold improvement of the SNR can be achieved compared to frame scanning.

FOLDED FRAME SCANNING

This approach allows imaging of a confined area along a line. The shape of the selected regions can be straight or curved while the position is arbitrary. This advanced scanning method is useful for imaging just cell bodies in the different places of the specimen or following events along winding dendrites with their protrusions.

TIME-LAPSE IMAGING

While two-photon excitation ensures depth penetration and fine spatial resolution, the high frame scanning rate of the resonant scanner ensures temporal resolution allowing observation of rapid events in living cells, neuronal networks or other circuits.

3D VOLUME SCANNING

Two new upgrade modules support near real-time, XYZ-T imaging of 3D volumes:

Both solutions combined with the fast frame scanning feature of resonant scanner allow fast, near real time scanning of a selected 3D volume.

APPLICATIONS

DEEP BRAIN IMAGING

Thanks to the two-photon laser technology and our optical developments, you can study nervus system with distinguishable cell bodies and dendrites down to 850 µm depth in the brain of behaving animals without photodamage.

CALCIUM IMAGING

To measure changes in Ca2+-levels is a powerful means for monitoring the activity of neural circuits. Calcium-indicators such as OGB-1 fluorescent dye or GCaMP fluorescent molecule can respond to the binding of Ca2+-ions by changing their fluorescence properties. To follow changes, the two kinds of scanners give the following possibilities:
  • resonant scanner allows to perform rapid series of measurements revealing events on a large field,
  • and using galvo scanner, you can measure the Ca2+-level on small, predetermined regions with high signal-to-noise ratio.

PARALLEL ELECTROPHYSIOLOGY

Hardware and software tools help parallel two-photon imaging and electrophysiological recordings, which provides different aspects to study the neuronal cell and network activity.

SOFTWARE

INTEGRATION

MESc fully integrates the control of all hardware units in the microscope such as core components (scanner, PMTs), light path actuators, various sample stages, auxiliary digital and analog channels.

ROI SELECTION

Numerous software tools support many kinds of 2D scanning pattern generation with high flexibility: point, line, curved line and areas with various shape can be selected thereby improving the efficiency of imaging.

REAL-TIME DISPLAY
AND ANALYSIS

Integrated, quantitative intensity-based calculations allow following real-time fluorescence changes and simultaneous analysis.

UPGRADE