Femto2D product line involves our firstly developed microscopes that have been on the market for more than 10 years. It involves conventional, modular galvanometer and resonant based two-photon microscopes which are used for both in vivo and in vitro studies. Since 2007, more than 60 Femto2D systems have been installed and being worked around the world.
Femto2D series contains three basic two-photon microscopes: Femto2D-Galvo, Femto2D-Resonant and Femto2D-Dual. They have standard Olympus frame which can be equipped with any kind of extensions selected from FemtoS and Femto2D modules’ portfolio, including in vitro extension. All of the in vivo imaging capability demonstrated in the case of the FemtoS is implemented to these systems, all scanning modes and optical properties are the same as in the FemtoS members.
All galvanometrer based scanning modes and excellent optical properties of the FemtoS-Galvo had been established in this microscope. High accuracy and positioning freedom of the scanner are combined with the intelligent MES control software supporting flexible approaches for ROI creation.
The Femto2D-Resonant is the twin of the FemtoS-Resonant. Femtonics combines the high speed and high sensitivity imaging of living tissues in this scope, where the imaging speed reaches the 31 frames per second. The optomechanical quality is ensured by the established Femtonics’ technology.
The Femto2D-Dual microscope gives the ability to perform dual scanning using both galvo and resonant scanners in tandem. With galvo scanner you can zoom to tiny structures such as dendritic spines and jump quickly between these zoomed regions. In contrast, with resonant scanner you can capture images with high frame rate to follow rapid changes on the FOV.
Revealing subtle changes of the evoked signals based on the following achievements:
- the shortest possible optical path allows enhanced photon collection efficiency thanks to our patented travelling detector system,
- using the highest sensitivity GaAsP photomultipliers (quantum efficiency >40%) optimized for scattered photons,
- carefully chosen high quality filtersets.