OVERVIEW

Femto2D-Resonant microscope is the most appropriate choice for imaging of the entire field of view with high frame rates. In this microscope Femtonics combines the high speed and high sensitivity imaging of living tissues. Resonant scanner based raster scanning is ~5-fold faster for fast acquisition of the entire field-of-view compared to galvanometric based scanning.

HIGH SPEED SCANNING

The resonant scanner consists of a fast oscillating mirror for x-axis deflection and a galvanometer mirror for y-axis sweep. Thanks to the 8 kHz oscillating speed of the fast mirror, the microscope is capable of gathering images at 31 frames per second.


INCREASED DETECTION
EFFICIENCY

High sensitivity photon detection allows superior signal-to-noise ratio based on the following achievements:
  • the shortest possible optical path allows enhanced photon collection efficiency thanks to our patented travelling detector system,
  • using the highest sensitivity GaAsP photomultipliers (quantum efficiency >40%), which are optimized for scattered photons,
  • carefully chosen high quality filtersets.

MODULARITY

The modular nature of the microscope supports to extend it with many upgrade elements ensuring a future proof performance in all kinds of optical-physiology measurements (uncaging, optogenetics, parallel electrophysiology, etc.). Tell us the concept, we create it.

FULL SPECIFICATION

HIGH SCANNING RATE
WITHOUT IMAGE DISTORSION

The velocity of resonant scanner is non-linear, the speed is different in the central and the edges of the frame. In the Femto2D-Resonant, Pockells-cell limits scanning range to that portion where scanning velocity is near linear, avoiding photobleaching/photodamage at the two sides of the image. Scan electronics performs dynamic pixel dwelling for data linearization and to cancel image distorsions.

LOW -POWER TEMPORAL OVERSAMPLING (LOTOS)

Femto2D-Resonant scans with high velocity thereby distributing excitation to a larger area resulting in decreased photodamage. This idea, called LOTOS method (Varga et al, PNAS (2011), Chen et al, Nature (2012)) involves imaging at high frame rates at lower excitation energy per pixel. As a result, the microscope allows imaging even small dendritic segments within the brain of behaving animals, e.g. to record individual calcium transients processing whisker stimulation.

3D VOLUME SCANNING

Two new upgrade modules support near real-time, XYZ-T imaging of 3D volumes:

Both solutions combined with the fast frame scanning feature of resonant scanner allow fast, near real time scanning of a selected 3D volume.

DEEP BRAIN IMAGING

Thanks to the two-photon laser technology and our optical developments, you can study nervous system with distinguishable cell bodies and dendrites down to 850 µm depth in the brain of behaving animals without photodamage.

CALCIUM-IMAGING

To measure changes in Ca2+-levels is a powerful method for monitoring the activity of many cells in the brain. Calcium-indicators such as the OGB-1 fluorescent dye or the GCaMP fluorescent protein family respond to the binding of Ca2+-ions by changing their fluorescence properties. The fast frame scanning speed of Femto2D-Resonant allows following rapid changes in neurons and the neuropil.

TIME-LAPSE IMAGING

While two-photon excitation ensures depth penetration and fine spatial resolution, the high frame scanning rate of the resonant scanner ensures temporal resolution allowing observation of rapid events in living cells, neuronal networks or other circuits.

LONG-TIME MEASUREMENTS

The high frame scanning rate and the unlimited video streaming combined with automated measurements support long time studies such as following developmental stadiums of a zebrafish embryo.

3D VOLUME SCANNING

Beside 3D activity changes in morphology of cellular networks or the vasculature can be revealed using 3D volume scanning upgrade (Piezo objective positioner, focus tunable liquid lens). The fast XY-scanning and Z-movement ensure near real time measurement of a 3D volume.

PHOTOSTIMULATION

Whole field illumination kit enables stimulation of cell populations in different locations of a specimen while the resonant scanner allows simultaneous imaging of the evoked signals. The selective stimulation of the cells can be ensured by expressing photosensitive molecules such as channelrhodopsin or halorhodopsin (optogenetics).

OVERVIEW

MESc is a highly modular measurement control and analysis software developed to drive our Femto2D-Resonant microscope. It was designed in C++ programming language with day-to-day lab experience in the field of cellular and network imaging.

INTEGRATION

MESc fully integrates the control of all hardware units in the microscope such as core components (scanner, PMTs), light path actuators, various sample stages, auxiliary digital and analog channels.

FEATURES

  • finely tunable measurement parameters (resolution, duration, frame average),
  • automatic adjustment of dynamic pixel dwell time to avoid image distorsion,
  • real-time data display and analysis,
  • data can be exported to common image and video format.

REAL-TIME DISPLAY AND ANALYSIS

Integrated, quantitative intensity-based calculations allow following real-time fluorescence changes and simultaneous analysis.

OVERVIEW

OVERVIEW

Femto2D-Resonant microscope is the most appropriate choice for imaging of the entire field of view with high frame rates. In this microscope Femtonics combines the high speed and high sensitivity imaging of living tissues. Resonant scanner based raster scanning is ~5-fold faster for fast acquisition of the entire field-of-view compared to galvanometric based scanning.

HIGH SPEED SCANNING

The resonant scanner consists of a fast oscillating mirror for x-axis deflection and a galvanometer mirror for y-axis sweep. Thanks to the 8 kHz oscillating speed of the fast mirror, the microscope is capable of gathering images at 31 frames per second.


INCREASED DETECTION
EFFICIENCY

High sensitivity photon detection allows superior signal-to-noise ratio based on the following achievements:
  • the shortest possible optical path allows enhanced photon collection efficiency thanks to our patented travelling detector system,
  • using the highest sensitivity GaAsP photomultipliers (quantum efficiency >40%), which are optimized for scattered photons,
  • carefully chosen high quality filtersets.

MODULARITY

The modular nature of the microscope supports to extend it with many upgrade elements ensuring a future proof performance in all kinds of optical-physiology measurements (uncaging, optogenetics, parallel electrophysiology, etc.). Tell us the concept, we create it.

FULL SPECIFICATION

TECHNOLOGY

HIGH SCANNING RATE
WITHOUT IMAGE DISTORSION

The velocity of resonant scanner is non-linear, the speed is different in the central and the edges of the frame. In the Femto2D-Resonant, Pockells-cell limits scanning range to that portion where scanning velocity is near linear, avoiding photobleaching/photodamage at the two sides of the image. Scan electronics performs dynamic pixel dwelling for data linearization and to cancel image distorsions.

LOW -POWER TEMPORAL OVERSAMPLING (LOTOS)

Femto2D-Resonant scans with high velocity thereby distributing excitation to a larger area resulting in decreased photodamage. This idea, called LOTOS method (Varga et al, PNAS (2011), Chen et al, Nature (2012)) involves imaging at high frame rates at lower excitation energy per pixel. As a result, the microscope allows imaging even small dendritic segments within the brain of behaving animals, e.g. to record individual calcium transients processing whisker stimulation.

3D VOLUME SCANNING

Two new upgrade modules support near real-time, XYZ-T imaging of 3D volumes:

Both solutions combined with the fast frame scanning feature of resonant scanner allow fast, near real time scanning of a selected 3D volume.

APPLICATIONS

DEEP BRAIN IMAGING

Thanks to the two-photon laser technology and our optical developments, you can study nervous system with distinguishable cell bodies and dendrites down to 850 µm depth in the brain of behaving animals without photodamage.

CALCIUM-IMAGING

To measure changes in Ca2+-levels is a powerful method for monitoring the activity of many cells in the brain. Calcium-indicators such as the OGB-1 fluorescent dye or the GCaMP fluorescent protein family respond to the binding of Ca2+-ions by changing their fluorescence properties. The fast frame scanning speed of Femto2D-Resonant allows following rapid changes in neurons and the neuropil.

TIME-LAPSE IMAGING

While two-photon excitation ensures depth penetration and fine spatial resolution, the high frame scanning rate of the resonant scanner ensures temporal resolution allowing observation of rapid events in living cells, neuronal networks or other circuits.

LONG-TIME MEASUREMENTS

The high frame scanning rate and the unlimited video streaming combined with automated measurements support long time studies such as following developmental stadiums of a zebrafish embryo.

3D VOLUME SCANNING

Beside 3D activity changes in morphology of cellular networks or the vasculature can be revealed using 3D volume scanning upgrade (Piezo objective positioner, focus tunable liquid lens). The fast XY-scanning and Z-movement ensure near real time measurement of a 3D volume.

PHOTOSTIMULATION

Whole field illumination kit enables stimulation of cell populations in different locations of a specimen while the resonant scanner allows simultaneous imaging of the evoked signals. The selective stimulation of the cells can be ensured by expressing photosensitive molecules such as channelrhodopsin or halorhodopsin (optogenetics).

SOFTWARE

OVERVIEW

MESc is a highly modular measurement control and analysis software developed to drive our Femto2D-Resonant microscope. It was designed in C++ programming language with day-to-day lab experience in the field of cellular and network imaging.

INTEGRATION

MESc fully integrates the control of all hardware units in the microscope such as core components (scanner, PMTs), light path actuators, various sample stages, auxiliary digital and analog channels.

FEATURES

  • finely tunable measurement parameters (resolution, duration, frame average),
  • automatic adjustment of dynamic pixel dwell time to avoid image distorsion,
  • real-time data display and analysis,
  • data can be exported to common image and video format.

REAL-TIME DISPLAY AND ANALYSIS

Integrated, quantitative intensity-based calculations allow following real-time fluorescence changes and simultaneous analysis.

UPGRADE