FemtoSmart is the first member of our next-generation two-photon microscope series. In the design, the main goal was to create a totally customizable microscope for in vivo studies with a distinct, charismatic appearance. The special feature of this microscope is the elevated body, which allows extremely free positioning of the sample below the objective.
The column-based X-Y-Z moving body houses the scanner unit, control circuits, and internal light path. The footprint of the leg is only 250 mm x 250 mm and there is a large space below the objective. The design enables the objective to move in a 50 mm range in the Z direction and the microscope can move in an XY direction around the base in 50 mm range. The tilting objective can further increase mobility.
The large space under the objective, where you can place virtual reality, movable tables, electrophysiological devices, is extremely useful for behavioral studies and supports functional studies of model organisms from C. elegans to non-human primates.
The modular nature of the microscope allows us to assemble the components, recombine and upgrade the system perfectly fitting the customer’s needs. One or two laser beams can be coupled to the microscope, a femtosecond, a two-photon laser for imaging and a secondary laser, either for imaging or photoactivation. Galvano or resonant scanner directs the laser beam, and dual scanner configuration is available too. The detection is established by multialkali and/or high-sensitivity GaAsP photomultipliers. The system can be extended with a lot of special devices such as a piezo objective positioner and a focus tunable liquid lens for fast Z-scanning, SmartBridge for increased mobility, etc. We offer special solutions for optogenetics and uncaging studies.
The design of this new microscope body is based on the established technology of Femtonics. As a result, the fine-tuned optical construction allows imaging in 850 µm depth on a wide field of view. The shortest optical path (patented) between the sample and the detectors ensures maximal photon collection efficiency and highest SNR. All scanning modes and excellent optical properties of the Femto2D-Galvo and Femto2D-Resonant microscopes are met in this microscope (see detailed specification on these pages).