OVERVIEW

FemtoSmart is the first member of our next-generation two-photon microscope series. In the design, the main goal was to create a totally customizable microscope for in vivo studies with a distinct, charismatic appearance. The special feature of this microscope is the elevated body, which allows extremely free positioning of the sample below the objective.

POSITIONING FREEDOM

The column-based X-Y-Z moving body houses the scanner unit, control circuits, and internal light path. The footprint of the leg is only 250 mm x 250 mm and there is a large space below the objective. The design enables the objective to move in a 50 mm range in the Z direction and the microscope can move in an XY direction around the base in 50 mm range. The tilting objective can further increase mobility.

IN VIVO STUDIES

The large space under the objective, where you can place virtual reality, movable tables, electrophysiological devices, is extremely useful for behavioral studies and supports functional studies of model organisms from C. elegans to non-human primates.

THE HIGHEST LEVEL OF MODULARITY

The modular nature of the microscope allows us to assemble the components, recombine and upgrade the system perfectly fitting the customer’s needs. One or two laser paths can be built into the microscope, a femtosecond, a two-photon laser for imaging and a secondary laser, either for imaging or photoactivation. Galvano or resonant scanner directs the laser beam, and dual scanner configuration is available too. The detection is established by multialkali and/or high-sensitivity GaAsP photomultipliers. The system can be extended with a lot of special devices such as a piezo objective positioner and a focus tunable liquid lens for fast Z-scanning, SmartBridge for increased mobility, etc. We offer special solutions for optogenetics and uncaging studies.

PROPERTIES OF IMAGING

The design of this new microscope body is based on the established technology of Femtonics. As a result, the fine-tuned optical construction allows imaging in 850 µm depth on a wide field of view. The shortest optical path (patented) between the sample and the detectors ensures maximal photon collection efficiency and highest SNR. All scanning modes and excellent optical properties of the Femto2D-Galvo and Femto2D-Resonant microscopes are met in this microscope (see detailed specification on these pages).

OVERVIEW

FemtoS Bridge is a special edition of the FemtoSmart microscope which is manufactured to have for extreme freedom in positioning of the body. It involves all advantages of the FemtoSmart series but it is extended to studies which need huge place for the sample or accessories therefore allowing functional brain imaging in head fixed mice navigating in a virtual reality environment.

THE ELEVATOR

In this FemtoS Bridge microscope, the foot is replaced with an lifting apparatus that provides an adjustable space between the microscope base and the top lens of the objective. The role of the modified elevating foot is to move the head along vertical direction. The moving range is 50 cm, the coarse Z-adjustment along the column is 1 mm. This elevator is situated outside of the workplace allowing an enlarged place below the Smart head. We provide the optimal combination of multiple height and working distance choices to address most animal models.

ACCESSORIES FOR FINE MOTIONS

Beside of the large movement established by the elevator, the fine motions can be supported
  • by the objective holding arm in a 50 mm range of Z direction,
  • piezo objective positioner for moving the objective with fast Z positioning in several hundred micrometers ranges,
  • an XY actuator can move the body relative to the column in X and Y direction (+/-25 mm range, step size: 1 um),
  • finally our tilting objective can further increase the accessibility to the side parts of the sample.

VERSATILITY

The FemtoS bridge offers the larges choice of height permutations that addresses applications from virtual reality, treadmills both line and spherical versions to electrophysiology hardware. If you have special clearance needs, please contact us with the dimensions so we can customize the travel mechanism.

OVERVIEW

OVERVIEW

FemtoSmart is the first member of our next-generation two-photon microscope series. In the design, the main goal was to create a totally customizable microscope for in vivo studies with a distinct, charismatic appearance. The special feature of this microscope is the elevated body, which allows extremely free positioning of the sample below the objective.

POSITIONING FREEDOM

The column-based X-Y-Z moving body houses the scanner unit, control circuits, and internal light path. The footprint of the leg is only 250 mm x 250 mm and there is a large space below the objective. The design enables the objective to move in a 50 mm range in the Z direction and the microscope can move in an XY direction around the base in 50 mm range. The tilting objective can further increase mobility.

IN VIVO STUDIES

The large space under the objective, where you can place virtual reality, movable tables, electrophysiological devices, is extremely useful for behavioral studies and supports functional studies of model organisms from C. elegans to non-human primates.

THE HIGHEST LEVEL OF MODULARITY

The modular nature of the microscope allows us to assemble the components, recombine and upgrade the system perfectly fitting the customer’s needs. One or two laser paths can be built into the microscope, a femtosecond, a two-photon laser for imaging and a secondary laser, either for imaging or photoactivation. Galvano or resonant scanner directs the laser beam, and dual scanner configuration is available too. The detection is established by multialkali and/or high-sensitivity GaAsP photomultipliers. The system can be extended with a lot of special devices such as a piezo objective positioner and a focus tunable liquid lens for fast Z-scanning, SmartBridge for increased mobility, etc. We offer special solutions for optogenetics and uncaging studies.

PROPERTIES OF IMAGING

The design of this new microscope body is based on the established technology of Femtonics. As a result, the fine-tuned optical construction allows imaging in 850 µm depth on a wide field of view. The shortest optical path (patented) between the sample and the detectors ensures maximal photon collection efficiency and highest SNR. All scanning modes and excellent optical properties of the Femto2D-Galvo and Femto2D-Resonant microscopes are met in this microscope (see detailed specification on these pages).

FEMTO-S BRIDGE

OVERVIEW

FemtoS Bridge is a special edition of the FemtoSmart microscope which is manufactured to have for extreme freedom in positioning of the body. It involves all advantages of the FemtoSmart series but it is extended to studies which need huge place for the sample or accessories therefore allowing functional brain imaging in head fixed mice navigating in a virtual reality environment.

THE ELEVATOR

In this FemtoS Bridge microscope, the foot is replaced with an lifting apparatus that provides an adjustable space between the microscope base and the top lens of the objective. The role of the modified elevating foot is to move the head along vertical direction. The moving range is 50 cm, the coarse Z-adjustment along the column is 1 mm. This elevator is situated outside of the workplace allowing an enlarged place below the Smart head. We provide the optimal combination of multiple height and working distance choices to address most animal models.

ACCESSORIES FOR FINE MOTIONS

Beside of the large movement established by the elevator, the fine motions can be supported
  • by the objective holding arm in a 50 mm range of Z direction,
  • piezo objective positioner for moving the objective with fast Z positioning in several hundred micrometers ranges,
  • an XY actuator can move the body relative to the column in X and Y direction (+/-25 mm range, step size: 1 um),
  • finally our tilting objective can further increase the accessibility to the side parts of the sample.

VERSATILITY

The FemtoS bridge offers the larges choice of height permutations that addresses applications from virtual reality, treadmills both line and spherical versions to electrophysiology hardware. If you have special clearance needs, please contact us with the dimensions so we can customize the travel mechanism.