OVERVIEW

As an alternative to other commercially available products, Femtonics developed a more effective synthesis path for producing MNI-Glu. MNI-Glutamate is a biochemically masked, nitro-indoline derivative of the neurotransmitter glutamate. Its photocleavage and the following Glu-release (uncaging) simultaneously with two-photon imaging is a powerful opportunity to investigate the distribution of glutamaterg receptors on the neurons. Our product is a different, biocompatible salt called MNI-Glu-TFA.

  • name: 4-methoxy-7-nitroindolinyl-L-glutamate trifluoroacetate
  • molecular formula: C16H18F3N3O8
  • mw: 437.32
  • standard packaging size: 13.5 mg
The caged compound exists as trifluoroacetic acid salted form (MNI-Glu*TFA), the TFA salt of compound ensures good solubility, stability and low hygroscopicity of the complex.

ADVANTAGES

  • resistant to hydrolysis
  • caged form has no interaction with neuronal receptors
  • patented material and synthesis
  • stable, soluble, low hygroscopicity
  • in complex with TFA
  • cheaper than alternatives


Figure: Photoactivation of MNI-Glu close to a dendritic segment of a hippocampal interneuron by Femto2D-Galvo.

REFERENCES

Correlated Synaptic Inputs Drive Dendritic Calcium Amplification and Cooperative Plasticity during Clustered Synapse Development, Kevin F.H. Lee, Cary Soares, Jean-Philippe Thivierge, Jean-Claude Béïque, Neuron (2016)