OVERVIEW

MESc is a second generation modular measurement control and analysis software driving our Femto2D-Resonant and FemtoSmart-Resonant microscopes. It was developed from the ground up to be able to squeeze the last bits of computing power from your PC to eliminate all software imposed limits on the size and complexity of your data. It is written in C++ programming language with the day-to-day lab experiences in the field of cellular and network imaging.

INTEGRATION

MESc fully integrates the control of all hardware units in the microscope as components, scanner, PMTs, light path actuators, auxiliary digital and analog channels, etc.

FEATURES

MESc is a user-friendly, convenient software for fast control and automation of measurements, real-time data display and analysis, and efficient batch processing of the huge amounts of measurement data.
MESc is capable of performing all the standard microscopy functions:
  • fully and finely tunable measurement parameters (resolution, duration, frame average),
  • automate setting of dynamic pixel dwell time to avoid image distorsion,
  • real-time data display and analysis,
  • unlimited video streaming,
  • data export to common image and video format.

REAL-TIME DISPLAY AND ANALYSIS

MESc uses an all-in-one-window concept to let you find everything you need in one place. To help your navigation, it also contains task-oriented views for measurement and data analysis. Integrated, quantitative intensity-based calculations allow to follow real-time fluorescence changes and simultaneous analysis.

ROI SELECTION

ROIs as polygons or rectangles can be selected and during the measurement their average fluorescence can be plotted in real time as the measurement is running.

VOLUME SCANNING

MESc offers two opportunities for controlling the scanning of a volume. In frame by frame Z ramping mode the fast objective positioner (usually a piezo objective positioner) takes a step between each plane, and as result, parallel planes are scanned. In continuous Z ramping mode the fast objective positioner steps between each scanline, and as a result, slightly tilted planes are scanned. This scanning mode enables faster volume scanning rates for volumetric physiology imaging.

LUT EDITING

MESc gives an opportunity to visualize the measurement data in color look-up table (LUT). LUT is a mechanism used to transform a range of values into a range of colors. LUTs come handy when displaying image data on the screen: by manipulating the LUTs of image channels, you can enhance the visibility of the important features of your measurement data.

DATA PROCESSING

Processing mode of the MESc enables you to open existing .mesc files, display and manipulate their contents and perform a few data processing steps. Each .mesc file stores a digital lab book which consists of a series of measurements (or measurement units) of different types. .mesc files are of virtually unlimited size, thus posing no size limits on your digital lab books (except of course the storage limits of your computing environment).