Two-photon Ca2+ imaging and electrophysiological data acquisition provide excellent tools for the study of neural cell and network activity. Using the two methods simultaneously, it is possible to follow potential fluctuations and relate them to changes in their Ca2+ levels. While the two-photon Ca2+ imaging technique shows events in multiple small areas (distinguishable parts of dendrites and spines) at high spatial resolution (less than 1 µm) but low temporal resolution (>100 ms), electrophysiology can cover changes over larger areas (cells or extensive areas) at low spatial resolution (~150 µm), and the changes can be followed considerably faster (<1 ms).