• Holographic illumination

    for 3D photostimulation

    Discover all

Technology Overview

The holographic stimulation is an extremely flexible method which produces simultaneous illumination in variable shape and size of multiple regions in the three-dimensional sample by using diffractive spatial light modulator (SLM).


  • 3D pattern activation in a 350×400×400 µm3 stimulating volume

  • simultaneous excitation in several depths with many ROIs by using SLM

  • ROIs are defined as polygons with arbitrary shape and size

  • high temporal resolution of the activation

  • lateral resolution less than 3 µm, axial resolution less than 10 µm (with 20x objective at 1040 nm)

  • excitation wavelength range: 1020-1080 nm


  • photostimulation of neurons by activating light sensitive ion channels such as ChR2 or NpHR

  • large illumination region allows activation of sufficient number of photoactive molecules

Optional modules

Femtonics Technology