OVERVIEW

The Tilting objective ​module provides ​a higher ​degree of freedom in moving ​the objective​ at precise angles, thereby it is ​ideally useful for in vivo experiments, intravital imaging, deep brain imaging in rodents or even non human primates.

The unit is driven by two motors which rotate the objective around horizontal and vertical axes. The movements are controlled by the software and by the handwheel unit.

SPECIFICATION

  • rotation around horizontal axis: 180º
  • rotation around vertical axis: 100º
  • movement with piezo: up to 400 µm (recommended)
  • speed of rotation: 4º/sec
  • maximum number of mounted detectors: 3
  • unidirectional repeatability < 0.02 mrad
  • bidirectional repeatability < 1 mrad
  • accepts both conventional and large back aperture objectives​
Can be equipped to all members of Femto2D product line (Femto2D-Galvo, Femto2D-Resonant and Femto2D-Dual) and FemtoS series.

BENEFITS

  • flexible objective positioning with high precision
  • motorized, software controlled
  • backlash-free, high stability at all positions
  • minimal dispersion, no optical aberration
  • minimized optical light path, detectors equipped to tilting unit
  • optional: fast movement of the objective in Z-direction with Piezo objective positioner
  • optional green lamp illumination

DEEP IN VIVO IMAGING OF SUBCORTICAL STRUCTURES

Novel surgical methods utilizing grin lenses or miniature prisms allow optical investigation of deep structures within the brain. Implementing these technologies is greatly enhanced by our motorized tilting objective:
  • aligning optical axis of the embedded optical elements is facilitated by the handwheel opetated multi-axis positioning of the grin lens/Smart microscope,
  • store and reuse optimal angles and positions when experimenting multiple times with the same animal,
  • utilize the units high-precision movements to fine adjust position and alignment without actually touching the assembly.