Atlas combines an intoxicating blend of high-tech science, engineering, refinement in 3D measurements.
The FEMTOSmart series is the next step in classical two-photon microscopy at Femtonics, fully customizable two-photon microscopes.
for three-photon excitation
Three-photon (3P) microscopy is a powerful technique that allows for the noninvasive structural and functional imaging of deep tissues with higher axial resolution compared to two-photon excitation. 3P excitation is performed at longer excitation wavelengths which scatter less in biological tissues. (See more at 3P imaging.) This extends the penetration depth, reduces out-of-focus excitation, and increases the SNR.
The figure shows spontaneous neuronal activity in the GCaMP6f-labeled visual cortex of a mouse captured using 3P microscopy: the cells were excited using a 1300 / 1400 nm laser wavelength for 3P excitation.
The 3P range microscopy kit contains:
for full-field photostimulation
Full-field illumination using a selected LED source allows molecules and cells to be stimulated over the whole FOV homogeneously. Combine this module with gated detectors to achieve millisecond switching between stimulation and imaging.
See more at Optogenetics
for vessel pattern visualization
Green illumination from an LED light source allows high-contrast visualization of blood vessels, taking advantage of that the highly concentrated heme content of the red blood cells is excitable in the spectral region of 300-650 nm. Using green illumination helps to navigate on the surface of any organs under in vivo conditions and position a patch pipette for bulk loading or patch clamping.
for free rotation of the objective
The motorized tilting module rotates the objective, giving a higher level of freedom to reach the sample from different angles. The module also includes a Piezo objective positioner, ensuring additional movement of the objective in the Z direction.
Novel surgical methods utilizing grin lenses or miniature prisms allow optical investigation of deep structures within the brain. Implementing these technologies is greatly enhanced by our motorized tilting objective:
for fast Z-stack and 3D imaging
A Piezo objective positioner kit enables the microscope to change the focal point by mechanically moving the objective. With this module, the microscope is able to collect signals from different depths fast enough to resolve biological activity in 3D samples. We can equip most of our microscopes with two types of piezo: piezo specialized for large scanning volumes moves the objective in a 400 µm travel range with up to 30 Hz speed, and the faster one moves it in 100 µm volume with up to 100 Hz (the refresh rate depends on the scanning volume and the size of the objective).
for coarse Z movement
The Bridge structure is a lifting apparatus for FemtoSmart which can replace the foot. It provides extreme freedom in positioning of the body and an increased space under the objective.
for multicolor full-field illumination
Wavelength-specific full-field excitation is performed by powerful LED light sources built in a rotating unit above the objective. Fluorescent imaging can be achieved with a wide variety of scientific cameras.
for Fluorescence Lifetime Imaging
Time correlated single photon counting and the derived fluorescence lifetime imaging measures the time delay between each emitted photon and the laser pulse eliciting it. This time is not affected by fluorophore concentration and excitation intensity fluctuations, however provides intimate information about molecular interactions and dynamics. See more: Applications – FLIM.
Fluorescence lifetime imaging reveals regulation of presynaptic Ca2+ by glutamate uptake and mGluRs, but not somatic voltage in cortical neurons. Olga Tyurikova, Kaiyu Zheng, Elizabeth Nicholson, Yulia Timofeeva, Alexey Semyanov, Kirill Volynski, Dmitri A. Rusakov, Journal of Neurochemistry (2020)
Local Resting Ca2+ Controls the Scale of Astroglial Ca2+ Signals. Claire M. King, Kirsten Bohmbach, Daniel Minge, Andrea Delekate, Kaiyu Zheng, James Reynolds, Cordula Rakers, Andre Zeug, Gabor C. Petzold, Dmitri A. Rusakov, Christian Henneberger, Cell Reports (2020)
Multiplex imaging relates quantal glutamate release to presynaptic Ca2+ homeostasis at multiple synapses in situ. Thomas P. Jensen, Kaiyu Zheng, Nicholas Cole, Jonathan S. Marvin, Loren L. Looger, Dmitri A. Rusakov, Nature Communications (2019)
Polymer microchamber arrays for geometry-controlled drug release: a functional study in human cells of neuronal phenotype. Olga Kopach, Kayiu Zheng, Olga A. Sindeeva, Meiyu Gai, Gleb B. Sukhorukov, Dmitri A. Rusakov, Biomaterials Science (2019)
Glutamate Imaging Reveals Multiple Sites of Stochastic Release in the CA3 Giant Mossy Fiber Boutons. Sylvain Rama, Thomas P. Jensen, Dmitri A. Rusakov, Front. Cell. Neurosci. (2019)
A genetically encoded fluorescent sensor for in vivo imaging of GABA. Jonathan S. Marvin, Yoshiteru Shimoda, Vincent Magloire, Marco Leite, Takashi Kawashima, Thomas P. Jensen, Ilya Kolb, Erika L. Knott, Ondrej Novak, Kaspar Podgorski, Nancy J. Leidenheimer, Dmitri A. Rusakov, Misha B. Ahrens, Dimitri M. Kullmann & Loren L. Looger, Nature Methods (2019)
Monitoring intracellular nanomolar calcium using fluorescence lifetime imaging. Kaiyu Zheng, Thomas P Jensen & Dmitri A Rusakov, Nature Protocols (2018)
Monitoring single-synapse glutamate release and presynaptic calcium concentration in organised brain tissue. Jensen TP, Zheng K, Tyurikova O, Reynolds JP, Rusakov DA, Cell Calcium (2017)
Time-Resolved Imaging Reveals Heterogeneous Landscapes of Nanomolar Ca2+ in Neurons and Astroglia. Kaiyu Zheng, Lucie Bard, James P. Reynolds, Claire King, Thomas P. Jensen, Alexander V. Gourine, Dmitri A. Rusakov, Neuron (2015)
for cultured cells or acute brain slices
Imaging acute brain slices or cultured cells and tissues enables the user to study cells in a controlled environment, outside of a living organism. Gradient contrast illumination eases camera guided patch-clamping while transmitted fluorescence detectors enhance signal collection and SNR.
Features:
Benefits:
for uncaging and optogenetics
The optomechanical design of the light path enables us to direct more beams into the microscope, utilizing the same light path. We offer secondary, fine-tuned laser sources for a wide range of biophotonics applications. See more: Optogenetics, Uncaging.
The FEMTO3D Atlas software:
Find 3D scanning modes and other features on the Atlas Software product page.
The FEMTOSmart software:
to monitor CO2 pressure
CO2 incubator is a device used to grow and nurture microbiological cultures or cell cultures. The incubator maintains optimal temperature, humidity, furthermore it evenly maintains and regulates the carbon dioxide content of the atmosphere inside. The device is essential for many kinds of research projects in various fields of science, such as cell biology, microbiology and molecular biology.
*Special promotion item.
for rodents
Changing the different types of stereotaxic instruments and adaptors enables you to perform a plathora of animal experiments, such as neurological disease experiments (Parkinson or Alzheimer’s disease, craniocerebral injury, spinal cord injury, etc.), brain drug injection, cannula implantation, physiological experiments (such as nerve stimulation, signal recording, wireless telemetry, etc.), microdialysis experiments (probe implantation and short-term dialysis) and many others.
The device is compatible with mouse and rat models.
for rodents
The experimental system is equipped with a gas recovery system to provide a safe experimental environment and to maximize the protection of the operator.
Shape the product to your needs: replacing the different types of anesthesia devices and masks enables you to perform different types of animal experiments, such as creating cerebral ischemia and myocardial ischemia models, osteoporosis models, performing tail vein injection, abdominal aorta blood sampling, heart blood sampling, ventricular injection, tissue / organ removal and other types of surgeries for your experiments.
The device is compatible with mouse and rat models.
*Special promotion item.
A blood perfusion imager based on the Laser Speckle Contrast Imaging (LSCI) technology. LSCI is a non-invasive optical imaging method used to obtain blood flow information in a wide rnage of applications, in ways that were not possible in the past. It allows visualization of tissue blood perfusion and imaging with high temporal (up to 140 fps) and spatial (2048×2048 pixels) resolution. The imager does not interfere with tissue perfusion, as no contact with the tissue is needed, and no dyes or tracer elements are neccessary.
*Special promotion item.
Aimed at detecting the activity change of neurons in the central nervous system in real-time, fiber photometry sums up the overall fluorescence of neurons to express their summed activity. Based on the reference channel of 410 nm, the RWD Fiber Photometry System adopts 470 nm and 560 nm dual-wavelength excitation, reducing background noise effectively. The significance of the reference channel lies in increasing self-control, reducing spontaneous fluorescence, eliminating signal artifacts caused by the animal’s intense movement and fiber winding, expressing two kinds of proteins at the same site, detecting levels of calcium change in different groups of neurons, and truly realizing the recording and analysis of dual-color fluorescence signals.
*Special promotion item.
Impactors are unique tools used for the induction of traumatic brain injury in animal models. They allow the experimenters to create a neurotrauma model with a high degree of reproducibility when it comes to the direction of impact and position of the injury. The pneumatically driven stereotaxic impactor is the most promising tool used for controlled cortical impact modeling: it delivers a precise and accurate injury to neocortex to mimic the significant physiological, histological, and behavioral aspects of traumatic brain injury and closed head injury. Choose from several user-friendly models, adaptable to various animal models with rodent or monkey adapters.
*Special promotion item.
Animal ventilators are the most common instruments for assisting animal respiration and are widely used by researchers, in preclinical and clinical medicine, in veterinary medicine, respiratory management during animal surgery and emergency treatment. This smart ventilator was designed for animal research applications and is widely using on subjects ranging in size from mice to guinea pigs. The ventilator provides to maintain versatility and ease of use while fostering safe and physiologically accurate mechanical ventilation conditions.
Features and benefits:
*Special promotion item.
The automated cell counter is a reliable cell counting devices with its highly intelligent analysis software and excellent microscopy optics structure. It can accurately identify the live cells, dead cells and the ones with specific fluorescence in a few seconds, which may liberate researchers from heavy work of daily cell counting.
*Special promotion item.
for craniotomy and brain positioning
An automatic and intelligent brain stereotaxic instrument, for craniotomy and precise positioning of brain regions in animal models. Compared to traditional manual positioners, the electric positioner can automatically calibrate and identify Bregma points, record and store multiple coordinate points, and display probe position in real time. With a resolution of 1μm.
*Special promotion item.
for light-controlled neuromodulation
The unmatched spatiotemporal precision and high tunability make optogenetics a powerful tool in neural research. This device integrates light source output and control into one tool, which is simpler and provides a more stable performance than other solutions. It comes with an intelligent design. Fields of application include research of neural circuits, learning and memory research, research of movement, addiction, sleep disorders, depression, anxiety, and Parkinson’s disease.
*Special promotion item.
for preparing tissue sections
Tissue sectioning made intelligent, precise, easy, and safe: this device provides reproducible, thin, serial sections of high quality. Comes with a patented visual pointer for quick and accurate section positioning. With a section thickness set range of 0,5-100µm.
*Special promotion item.
*Special promotion item.
*Special promotion item.
for rodents
Head holder stands and head plates fix the rodent’s head in different positions, enabling precise measurements in the brain. Three types of head holders are available with different dimensions. For anesthetized rodents, we offer a heating pad coupled to a holder, or for behaving animals a stand that ensures access to jetballs, treadmills, or other devices. See more at Behavioral Studies.
Offering maximum flexible adjustment for positioning the head, this holder fixes the scull from one side. The head plate can be moved in three directions of the space and also along the bridge. It is offered for difficult to access parts such as eyes or auditory cortex, and anesthetized rodents.
Stabilized head holder is attached to the rodent’s head at two sides. It is ideal for craniotomy or other surgery where the head of the animal model has to be fixed. It can be coupled to the Gramophone.
Technology Overview
This dinitro-indoline-masked form of glutamate releases the bioactive glutamate more rapidly than any other commercially available compound. It was developed for high-quantum yield requiring less irradiation for release, so its effective concentration is lower than other caging scaffolds. The caged compound exists as trifluoroacetic acid salted form (DNI-Glu*TFA) ensuring good solubility, stability and low hygroscopicity. DNI-Glu is a compound developed in-house, only available from Femtonics.
See more at Uncaging.
Endocannabinoid Signaling Mediates Local Dendritic Coordination between Excitatory and Inhibitory Synapses.
Hai Yin Hu, Dennis L.H. Kruijssen, Cátia P. Frias, Balázs Rózsa, Casper C. Hoogenraad, Cell Reports (2019)
A compact holographic projector module for high-resolution 3D multi-site two-photon photostimulation.
Mary Ann Go, Max Mueller, Michael Lawrence Castañares, Veronica Egger, Vincent R. Daria, PLOS One (2019)
Coincidence Detection within the Excitable Rat Olfactory Bulb Granule Cell Spines.
S. Sara Aghvami, Max Müller, Babak N. Araabi and Veronica Egger, J. Neurosci. (2019)
Voltage Gated Calcium Channel Activation by Backpropagating Action Potentials Downregulates NMDAR Function
Anne-Kathrin Theis, Balázs Rózsa, Gergely Katona, Dietmar Schmitz and Friedrich W. Johenning, Front. Cell. Neurosci. (2018)
High efficiency two-photon uncaging coupled by the correction of spontaneous hydrolysis.
Dénes Pálfi, Balázs Chiovini, Gergely Szalay, Attila Kaszás, Gergely F. Turi, Gergely Katona, Péter Ábrányi-Balogh, Milán Szőri, Attila Potor, Orsolya Frigyesi, Csilla Lukácsné Haveland, Zoltán Szadai, Miklós Madarász, Anikó Vasanits-Zsigrai, Ibolya Molnár-Perl, Béla Viskolcz, Imre G. Csizmadia, Zoltán Mucsi and Balázs Rózsa, Org. Biomol. Chem. (2018)
Super-Resolution Imaging of the Extracellular Space in Living Brain Tissue
Jan Tønnesen, V.V.G. Krishna Inavalli, U. Valentin Nägerl, Cell (2018)
Imaging membrane potential changes from dendritic spines using computer-generated holography.
Dimitrii Tanese, Ju-Yun Weng, Valeria Zampini, Vincent de-Sars, Marco Canepari, Balazs J. Rozsa, Valentina Emiliani, Dejan Zecevic, Neurophotonics (2017)
Cell-type–specific inhibition of the dendritic plateau potential in striatal spiny projection neurons.
Kai Du, Yu-Wei Wu, Robert Lindroos, Yu Liu, Balázs Rózsa, Gergely Katona, Jun B. Ding, and Jeanette Hellgren Kotaleski, PNAS (2017)
Electrical behaviour of dendritic spines as revealed by voltage imaging.
Marko A. Popovic, Nicholas Carnevale, Balazs Rozsa; Dejan Zecevic, Nature Communications (2015)
Local Postsynaptic Voltage-Gated Sodium Channel Activation in Dendritic Spines of Olfactory Bulb Granule Cells.
Wolfgang G. Bywalez, Dinu Patirniche, Vanessa Rupprecht, Martin Stemmler, Andreas;V.M. Herz, Denes Palfi, Balazs Rozsa, Veronica Egger, Neuron (2015)
Quantitation of various indolinyl caged glutamates as their o-phthalaldehyde derivatives by high performance liquid chromatography coupled with tandem spectroscopic detections: derivatization, stoichiometry and stability studies.
Vasanits-Zsigrai A, Majercsik O, Toth G, Csampai A, Haveland-Lukacs C, Palfi D, Szadai Z, Rozsa B, Molnar-Perl I, J Chromatogr A. (2015)
Dendritic spikes induce ripples in parvalbumin interneurons during hippocampal sharp waves.
B Chiovini, G F Turi, G Katona, A Kaszas, D Palfi, P Maak, G Szalay, M F Szabo, Z Szadai, Sz Kali and B Rozsa, Neuron (2014)
This dinitro-indoline-masked form of glutamate releases the bioactive glutamate more rapidly than any other commercially available compound. It was developed for high-quantum yield requiring less irradiation for release, so its effective concentration is lower than other caging scaffolds. The caged compound exists as trifluoroacetic acid salted form (DNI-Glu*TFA) ensuring good solubility, stability and low hygroscopicity. DNI-Glu is a compound developed in-house, only available from Femtonics.
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Lorem ipsum dolor sit amet consectetur, adipisicing elit. Tenetur deleniti architecto, optio quibusdam cum, rerum, beatae molestiae eos saepe perspiciatis quos voluptates ad. Fugiat ducimus dignissimos non, illo eligendi esse?
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All of our knowledge and results of acousto-optic technology have been poured into our new product, the Femto3D Atlas which has become the peak of the evolution of Femtonics acousto-optic microscopes. Atlas combines an intoxicating blend of high-tech science, engineering, refinement in 3D measurements. Using Atlas, researchers are capable of scanning neuronal, dendritic, and other neuropil activities in 3D about one million times faster compared to classical scanning methods with preserved two-photon resolution thanks to a unique acousto-optical scanner and the superior optical design. Combining its unique fast 3D imaging feature with the property that it implements and goes beyond the traditional galvo and resonant scanner-based microscope imaging functions, Atlas provides an all-in-one solution into the scientists’ hand.
The FemtoSmart series is the next step in classical two-photon microscopy at Femtonics, fully customizable two-photon microscopes. Their special feature is the elevated body which can move in X, Y, and Z directions, providing ample room under the objective for optimal positioning of your sample. This feature makes them suitable for model organisms ranging from zebra fish larvae, through mice navigating in virtual reality to even non-human primates. The microscope’s modular nature allows us to assemble the components, and recombine and upgrade the system to perfectly fit the customer’s needs. FemtoSmart product line contains galvo and/or resonant scanner-based systems which can be equipped with a lot of optional modules enabling it to be adapted to a wide range of biological applications, such as optogenetics, uncaging and dendritic imaging.