Products

IN VITRO EXTENSION KIT

for cultured cells or acute brain slices

Technology Overview

Imaging acute brain slices or cultured cells and tissues enables the user to study cells in a controlled environment, outside of a living organism. Gradient contrast illumination eases camera guided patch-clamping while transmitted fluorescence detectors enhance signal collection and SNR.

Features:

• Transmission Köhler illumination with a high-power IR LED (850nm)
• High NA condenser (dry or oil immersion options)
• Visible light detection (Fluorescence, SHG, THG, CARS, etc): efficient, large aperture light collecting optics with visible detection ports between 400-690 nm, a maximum of 2 visible light detectors (options: GaAsP, gated GaAsP, multialkali PMT or hybrid detectors for FLIM), and a manually exchangeable filter cube
• Transmission infrared detector with a TIR detection port between 720-1070 nm (options: biased silicon photodiode or multialkali PMT)

Benefits:

• An easily replaceable protective window with waterproof sealing safeguards the sensitive internal optics from leaking water and corrosive solutions during experiments
• Versatile mechanical solutions:
• Compatible with Femtonics microscopes (SMART with tiltable or normal objective arm, Platform), and with an LN stage
• The condenser, condenser holder or the whole module is removable prior to in vivo measurements, owing to the quick release adapter placed on the fix mount to the optical table

PIEZO OBJECTIVE POSITIONER KIT

for fast Z-stack and 3D imaging

Technology Overview

A Piezo objective positioner kit enables the microscope to change the focal point by mechanically moving the objective. With this module, the microscope is able to collect signals from different depths fast enough to resolve biological activity in 3D samples. We can equip most of our microscopes with two types of piezo: piezo specialized for large scanning volumes moves the objective in a 400 µm travel range with up to 30 Hz speed, and the faster one moves it in 100 µm volume with up to 100 Hz (the refresh rate depends on the scanning volume and the size of the objective).

Features

• Positions objectives in the Z direction with nm resolution
• Traveling range 100 or 400 µm (depends of the piezo’s type)
• Up to 100 Hz frequency in resonant mode
• Millisecond step-and-settle
• Any types of objectives can be attached
• No influence on the optical path

Benefits

• Femtonics microscopes containing galvo scanner equipped with piezo objective positioner and RollerCoaster software module support 3D trajectory scanning
• 3D trajectory scanning allows collecting signals from dendrites arbored in the 3D tissue and to resolve biological activity thanks to the high scanning speed
• Femtonics microscopes containing resonant or galvo scanners are able to image a 3D volume scanning
• 3D volume scanning enables you to reveal the activity of the neural network or other cell groups
• Fine positioning along the tilted axis when using tilting objective module

MOTORIZED TILTING OBJECTIVE UNIT

for free rotation of the objective

Technology Overview

The motorized tilting module rotates the objective, giving a higher level of freedom to reach the sample from different angles. The module also includes a Piezo objective positioner, ensuring additional movement of the objective in the Z direction.

Applications

Novel surgical methods utilizing grin lenses or miniature prisms allow optical investigation of deep structures within the brain. Implementing these technologies is greatly enhanced by our motorized tilting objective:

• aligning optical axis of the embedded optical elements is facilitated by the handwheel operated multi-axis positioning of the grin lens/Smart microscope,
• store and reuse optimal angles and positions when experimenting multiple times with the same animal,
• utilize the units high-precision movements to fine adjust position and alignment without actually touching the assembly,
• suitable for imaging lateral brain regions, even in non-human primates.

Features

• 180º rotation around the horizontal axis
• 100º rotation around the vertical axis
• Speed of rotation: 4º/sec
• Unidirectional repeatability: less than 0.02 mrad (~2 µm)
• Z movement with piezo objective positioner module: 400 µm
• Transmission over 80% (700-1100 or 900-1300 nm)
• Accepts both conventional and large back aperture objectives
• Green illumination module
• Maximum number of mounted detectors: 3

Benefits

• Flexible objective positioning with high precision
• Highly stable in all positions
• Fast z-stack acquisition also from tilted position
• Close coupled detectors
• Useful for in vivo experiments, intravital imaging, and deep brain imaging in rodents or even non-human primates

EPIFLUORESCENT UNIT

for multicolor full-field illumination

Technology Overview

Wavelength-specific full-field excitation is performed by powerful LED light sources built in a rotating unit above the objective. Fluorescent imaging can be achieved with a wide variety of scientific cameras.

Features

• LEDs for customizable wavelengths (430, 450, 480, 530, 590 nm, others on special request)
• Wavelength-specific filters for excitation of narrow ranges
• Additional emission filters for blocking the reflected light and filtering the emission spectra
• Up to 5 sets of exchangeable filter cubes
• 100 µm penetration depth
• Detection by CMOS camera
• Software controlled operation
• Installed into the light path above the objective
• PMT protection during the stimulation by built-in gating system

Benefits

• Homogenous excitation of the entire FOV in 100 µm depth
• Excitation specified for GFP, YFP, RFP, mCherry, etc.
• Cleared emission for bright signals

GREEN ILLUMINATION

for vessel pattern visualization

Technology Overview

Green illumination from an LED light source allows high-contrast visualization of blood vessels, taking advantage of that the highly concentrated heme content of the red blood cells is excitable in the spectral region of 300-650 nm. Using green illumination helps to navigate on the surface of any organs under in vivo conditions and position a patch pipette for bulk loading or patch clamping.

Features

• Excitation wavelength at 510-540 nm
• ~100 µm penetration depth
• 3 sec switching time between camera and two-photon modes
• Detection by CMOS camera
• Adjustable brightness, contrast and gamma parameters
• Software controlled operation
• Equipped on the objective arm
• Built-in PMT protection during operation

Benefits

• High-contrast visualization
• Precise overlap in XYZ between camera and two-photon recorded field of view
• Locate your chronic imaging spot within the craniotomy

LED LIGHT SOURCE

for full-field photostimulation

Technology Overview

Full-field illumination using a selected LED source allows molecules and cells to be stimulated over the whole FOV homogeneously. Combine this module with gated detectors to achieve millisecond switching between stimulation and imaging.

See more at Optogenetics

Features

• Typical wavelengths: 430, 450, 480, 590 nm, others on special request
• Precisely timed and highly repeatable impulses
• ~200 µm penetration depth
• Software controlled operation
• Equipped on the objective arm
• PMT protection during the stimulation by optional gated detectors

Benefits

• Simultaneous stimulation over the entire FOV
• Optimized for optogenetics studies: precise spatio-temporal activation of ChR2 and/or NpHR
• Millisecond switching between stimulation and detection

3P RANGE MICROSCOPY

for three-photon excitation

Technology Overview

The three-photon (3P) microscopy allows noninvasive functional imaging of the deeper tissues with higher axial resolution compared to the two-photon excitation. 3P excitation is performed at longer excitation wavelengths which are scattered less in the biological tissues. (See more at 3P imaging) This allows extending the penetration depth, reduces out-of-focus excitation, and increases the SNR.

The 3P range microscopy kit contains:

• Coherent Monaco pump laser and Opera-F optical parametric amplifier system
• High quality optical elements optimized transmission between 1,200-1,700 nm
• Full optical engineering

Features

• Excitation wavelength range:1,200-1,700 nm
• XY resolution is diffraction limited (less than 1 µm, wavelength dependent), Z resolution ~ 2 µm (sample and wavelength dependent)
• Excites fluorophores in the corresponding 1P excitation wavelength range from blue to green
• Less tissue damage compared to 2P excitation

Benefits

• Third Harmonic Generation (THG) option available
• Functional imaging through the whole cortical depth

LIQUID LENS OBJECTIVE KIT

for fast 3D imaging

Technology Overview

The Liquid lens objective kit contains a carefully chosen objective and a fast-focusing element that utilizes an electrically controlled shape-changing membrane. This flexible membrane enables the objective to switch quickly between focal planes.

Features

• Focuses with nanometer precision
• 200 µm focal range
• 10 ms focal-plane switching time
• Fast settling time
• Transmission 90% at 800 nm
• Aperture 10 mm
• Comes with a 40× magnification objective
• No mechanical perturbation
• 3D volume scanning

Benefits

• Fast switch between the focal planes
• 3D volume scanning enables you to reveal activity of the neural network or other cell groups
• Imaging at multiple tissue depths near simultaneously

BRIDGE STRUCTURE

for coarse Z movement

Technology Overview

The Bridge structure is a lifting apparatus for FemtoSmart which can replace the foot. It provides extreme freedom in positioning of the body and an increased space under the objective.

Features

• Moving range: 50 cm
• Coarse Z adjustment: 1 mm
• Distance between the foot and the objective: 880 mm

Benefits

• Very large space for the sample or accessories
• High-range mobility
• Locate your chronic imaging spot within the craniotomy

MULTIPLE BEAM PATH

for uncaging and optogenetics

Technology Overview

The optomechanical design of the light path enables us to direct more beams into the microscope, utilizing the same light path. We offer secondary, fine-tuned laser sources for a wide range of biophotonics applications. See more: Optogenetics, Uncaging.

Features

• Additional IR or CW laser(s) coupled to the existing light path
• Lightpath can be optimized for three wavelength ranges  (450-1100 or 700-1100 or 900-1300 nm)
• Full optical engineering
• Software controlled operation

Benefits

• Photostimulation with IR or visible light
• Flexible stimulation patterns supporting stimulation of selected regions
• Two-photon uncaging studies
• Visible light optogenetics:
  • ChR2 activation with 473 nm laser
  • NpHR activation with 561 nm laser

GRAMOPHONE

for behavioral studies            

Technology Overview

Gramophone is a single-dimension locomotion tracking device. It allows a head restrained mouse to run on the disk and to respond to visual or other stimuli. The speed and the direction of the running are registered by software-controlled manner. It can be used for high-accuracy velocity recording in conjunction with a two-photon microscope, or as a control interface for behavioral training in a virtual linear maze. The behavior response and the two-photon Ca²⁺ signals can be recorded simultaneously and aligned with each other.

Features

• 14400 counts/rotation angular resolution
• Up to 500 Hz sampling frequency
• Robust and durable construction
• Easily removable and cleanable running surface
• Easily adjustable head fixation for in vivo imaging and electrophysiology
• Programmable inputs and outputs
• Open-source Python 3 software for recording locomotion and conditioning mice

Benefits

• Flexible usability: mobile, compact system for behavioral training, locomotion measurements, microscopic imaging, electrophysiological studies, etc.
• Easily integratible to your own system
• Custom mazes and behavior rules can be designed easily
• Built-in image patterns
• Velocity and movement detection
• The system can handle two monitors simultaneously, providing a corridor-like arrangement
• Stabilized head holder keeps the skull stable during movement