Atlas combines an intoxicating blend of high-tech science, engineering, refinement in 3D measurements.
FEATURES AND BENEFITS
- 3D imaging extension for any existing microscopes and individual system
- Simultaneous read out of neural activity on both the somatic and dendritic scales
- Increased measurement speed and signal-to-noise ratio by several orders of magnitude
- 3D Network imaging of over 2000 soma distributed in 3D
- Imaging of dendritic branches through layers
- Simultaneous imaging of over thousands of spines
- Unique, flexible scanning methods supporting in vivo and in vitro measurements
- In vivo 3D motion correction by specialized scanning patterns
- High-speed 3D random-access ROI scanning: scanning speed up to 30 kHz to any points in 3D
- High-speed raster scanning: 40 fps at 510×510, and even 3000 fps at reduced FOV
- Improved excitation efficiency for large 3D scanning volume with GECIs
- Automatic wavelength tuning between 750 – 1050 nm
- Functional imaging correlated with electrophysiological data
- Calcium imaging during behavior
- 3D random-access photo stimulation for optogenetics
Femto
Smart
The FEMTOSmart series is the next step in classical two-photon microscopy at Femtonics, fully customizable two-photon microscopes.
FEATURES AND BENEFITS
- Elevated, column-based body
- XYZ positioning
- Tilting objective
- High level of modularity
- Galvanometric, resonant, or both scanning possibilities
- Galvo Scanner, focusing on regions-of-interest
- Resonant Scanner, fast frame and volume scanning
- Dual Scanner, all advantages of Galvo and Resonant microscopes
- Travelling detector system
- For in vivo studies
- Field upgradability
- Patented imaging technologies
- Flexible scanning methods
- Maximal photon collection
3P RANGE MICROSCOPY
for three-photon excitation
Technology Overview
Three-photon (3P) microscopy is a powerful technique that allows for the noninvasive structural and functional imaging of deep tissues with higher axial resolution compared to two-photon excitation. 3P excitation is performed at longer excitation wavelengths which scatter less in biological tissues. (See more at 3P imaging.) This extends the penetration depth, reduces out-of-focus excitation, and increases the SNR.
The figure shows spontaneous neuronal activity in the GCaMP6f-labeled visual cortex of a mouse captured using 3P microscopy: the cells were excited using a 1300 / 1400 nm laser wavelength for 3P excitation.
The 3P range microscopy kit contains:
- A pump laser and a Noncollinear Optical Parametric Amplifier (NOPA)
- High-quality optical elements optimized for transmission between 1,200-1,700 nm (with optional extension: get in contact with us for details)
- Full optical engineering
Features
- Excitation wavelength range: 1,200-1,700 nm
- XY resolution is diffraction-limited (less than 1 µm, wavelength-dependent), Z resolution ~ 5-10 µm (sample and wavelength-dependent)
- Excites fluorophores with the 3P excitation phenomenon: 3P wavelength range (1,200-1,700 nm) corresponds to the regular (1P) excitation wavelength range of fluorophores ranging from blue to red
- Less tissue damage compared to 2P excitation
Benefits
- Functional and structural imaging through the whole cortical depth; provides an advantage in strongly scattering samples
- Third Harmonic Generation (THG) option is available
GREEN ILLUMINATION
for vessel pattern visualization
Technology Overview
Green illumination from an LED light source allows high-contrast visualization of blood vessels, taking advantage of that the highly concentrated heme content of the red blood cells is excitable in the spectral region of 300-650 nm. Using green illumination helps to navigate on the surface of any organs under in vivo conditions and position a patch pipette for bulk loading or patch clamping.
Features
- Excitation wavelength at 510-540 nm
- ~100 µm penetration depth
- 3 sec switching time between camera and two-photon modes
- Detection by CMOS camera
- Adjustable brightness, contrast and gamma parameters
- Software controlled operation
- Equipped on the objective arm
- Built-in PMT protection during operation
Benefits
- High-contrast visualization
- Precise overlap in XYZ between camera and two-photon recorded field of view
- Locate your chronic imaging spot within the craniotomy
MOTORIZED TILTING OBJECTIVE UNIT
for free rotation of the objective
Technology Overview
The motorized tilting module rotates the objective, giving a higher level of freedom to reach the sample from different angles. The module also includes a Piezo objective positioner, ensuring additional movement of the objective in the Z direction.
Applications
Novel surgical methods utilizing grin lenses or miniature prisms allow optical investigation of deep structures within the brain. Implementing these technologies is greatly enhanced by our motorized tilting objective:
- aligning optical axis of the embedded optical elements is facilitated by the handwheel operated multi-axis positioning of the grin lens/Smart microscope,
- store and reuse optimal angles and positions when experimenting multiple times with the same animal,
- utilize the units high-precision movements to fine adjust position and alignment without actually touching the assembly,
- suitable for imaging lateral brain regions, even in non-human primates.
Features
- 180º rotation around the horizontal axis
- 100º rotation around the vertical axis
- Speed of rotation: 4º/sec
- Unidirectional repeatability: less than 0.02 mrad (~2 µm)
- Z movement with piezo objective positioner module: 400 µm
- Transmission over 80% (700-1100 or 900-1300 nm)
- Accepts both conventional and large back aperture objectives
- Green illumination module
- Maximum number of mounted detectors: 3
Benefits
- Flexible objective positioning with high precision
- Highly stable in all positions
- Fast z-stack acquisition also from tilted position
- Close coupled detectors
- Useful for in vivo experiments, intravital imaging, and deep brain imaging in rodents or even non-human primates
PIEZO OBJECTIVE POSITIONER KIT
for fast Z-stack and 3D imaging
Technology Overview
A Piezo objective positioner kit enables the microscope to change the focal point by mechanically moving the objective. With this module, the microscope is able to collect signals from different depths fast enough to resolve biological activity in 3D samples. We can equip most of our microscopes with two types of piezo: piezo specialized for large scanning volumes moves the objective in a 400 µm travel range with up to 30 Hz speed, and the faster one moves it in 100 µm volume with up to 100 Hz (the refresh rate depends on the scanning volume and the size of the objective).
Features
- Positions objectives in the Z direction with nm resolution
- Traveling range 100 or 400 µm (depends of the piezo’s type)
- Up to 100 Hz frequency in resonant mode
- Millisecond step-and-settle
- Any types of objectives can be attached
- No influence on the optical path
Benefits
- Femtonics microscopes containing galvo scanner equipped with piezo objective positioner and RollerCoaster software module support 3D trajectory scanning
- 3D trajectory scanning allows collecting signals from dendrites arbored in the 3D tissue and to resolve biological activity thanks to the high scanning speed
- Femtonics microscopes containing resonant or galvo scanners are able to image a 3D volume scanning
- 3D volume scanning enables you to reveal the activity of the neural network or other cell groups
- Fine positioning along the tilted axis when using tilting objective module
BRIDGE STRUCTURE
for coarse Z movement
Technology Overview
The Bridge structure is a lifting apparatus for FemtoSmart which can replace the foot. It provides extreme freedom in positioning of the body and an increased space under the objective.
Features
- Moving range: 50 cm
- Coarse Z adjustment: 1 mm
- Distance between the foot and the objective: 880 mm
Benefits
- Very large space for the sample or accessories
- High-range mobility
- Locate your chronic imaging spot within the craniotomy
EPIFLUORESCENT UNIT
for multicolor full-field illumination
Technology Overview
Wavelength-specific full-field excitation is performed by powerful LED light sources built in a rotating unit above the objective. Fluorescent imaging can be achieved with a wide variety of scientific cameras.
Features
- LEDs for customizable wavelengths (430, 450, 480, 530, 590 nm, others on special request)
- Wavelength-specific filters for excitation of narrow ranges
- Additional emission filters for blocking the reflected light and filtering the emission spectra
- Up to 5 sets of exchangeable filter cubes
- 100 µm penetration depth
- Detection by CMOS camera
- Software controlled operation
- Installed into the light path above the objective
- PMT protection during the stimulation by built-in gating system
Benefits
- Homogenous excitation of the entire FOV in 100 µm depth
- Excitation specified for GFP, YFP, RFP, mCherry, etc.
- Cleared emission for bright signals
FLIM EXTENSION
for Fluorescence Lifetime Imaging
Technology Overview
Time correlated single photon counting and the derived fluorescence lifetime imaging measures the time delay between each emitted photon and the laser pulse eliciting it. This time is not affected by fluorophore concentration and excitation intensity fluctuations, however provides intimate information about molecular interactions and dynamics. See more: Applications – FLIM.
Features
- High efficiency hybrid GaAsP PMTs for photon counting
- No afterpulsing
- Up to two photon counting wavelength channels
- Non-descanned design for high photon collection rate
- Minimized optical path length by travelling detector system
- Sub-picosecond temporal resolution
- Parallel conventional integrating and photon counting modes
Benefits
- Absolute ion concentration measurement
- Characterization of bio-physical properties
Related Publications:
Fluorescence lifetime imaging reveals regulation of presynaptic Ca2+ by glutamate uptake and mGluRs, but not somatic voltage in cortical neurons. Olga Tyurikova, Kaiyu Zheng, Elizabeth Nicholson, Yulia Timofeeva, Alexey Semyanov, Kirill Volynski, Dmitri A. Rusakov, Journal of Neurochemistry (2020)
Local Resting Ca2+ Controls the Scale of Astroglial Ca2+ Signals. Claire M. King, Kirsten Bohmbach, Daniel Minge, Andrea Delekate, Kaiyu Zheng, James Reynolds, Cordula Rakers, Andre Zeug, Gabor C. Petzold, Dmitri A. Rusakov, Christian Henneberger, Cell Reports (2020)
Multiplex imaging relates quantal glutamate release to presynaptic Ca2+ homeostasis at multiple synapses in situ. Thomas P. Jensen, Kaiyu Zheng, Nicholas Cole, Jonathan S. Marvin, Loren L. Looger, Dmitri A. Rusakov, Nature Communications (2019)
Polymer microchamber arrays for geometry-controlled drug release: a functional study in human cells of neuronal phenotype. Olga Kopach, Kayiu Zheng, Olga A. Sindeeva, Meiyu Gai, Gleb B. Sukhorukov, Dmitri A. Rusakov, Biomaterials Science (2019)
Glutamate Imaging Reveals Multiple Sites of Stochastic Release in the CA3 Giant Mossy Fiber Boutons. Sylvain Rama, Thomas P. Jensen, Dmitri A. Rusakov, Front. Cell. Neurosci. (2019)
A genetically encoded fluorescent sensor for in vivo imaging of GABA. Jonathan S. Marvin, Yoshiteru Shimoda, Vincent Magloire, Marco Leite, Takashi Kawashima, Thomas P. Jensen, Ilya Kolb, Erika L. Knott, Ondrej Novak, Kaspar Podgorski, Nancy J. Leidenheimer, Dmitri A. Rusakov, Misha B. Ahrens, Dimitri M. Kullmann & Loren L. Looger, Nature Methods (2019)
Monitoring intracellular nanomolar calcium using fluorescence lifetime imaging. Kaiyu Zheng, Thomas P Jensen & Dmitri A Rusakov, Nature Protocols (2018)
Monitoring single-synapse glutamate release and presynaptic calcium concentration in organised brain tissue. Jensen TP, Zheng K, Tyurikova O, Reynolds JP, Rusakov DA, Cell Calcium (2017)
Time-Resolved Imaging Reveals Heterogeneous Landscapes of Nanomolar Ca2+ in Neurons and Astroglia. Kaiyu Zheng, Lucie Bard, James P. Reynolds, Claire King, Thomas P. Jensen, Alexander V. Gourine, Dmitri A. Rusakov, Neuron (2015)
IN VITRO EXTENSION KIT
for cultured cells or acute brain slices
Technology Overview
Imaging acute brain slices or cultured cells and tissues enables the user to study cells in a controlled environment, outside of a living organism. Gradient contrast illumination eases camera guided patch-clamping while transmitted fluorescence detectors enhance signal collection and SNR.
Features:
- Transmission Köhler illumination with a high-power IR LED (850nm)
- High NA condenser (dry or oil immersion options)
- Visible light detection (Fluorescence, SHG, THG, CARS, etc): efficient, large aperture light collecting optics with visible detection ports between 400-690 nm, a maximum of 2 visible light detectors (options: GaAsP, gated GaAsP, multialkali PMT or hybrid detectors for FLIM), and a manually exchangeable filter cube
- Transmission infrared detector with a TIR detection port between 720-1070 nm (options: biased silicon photodiode or multialkali PMT)
Benefits:
- An easily replaceable protective window with waterproof sealing safeguards the sensitive internal optics from leaking water and corrosive solutions during experiments
- Versatile mechanical solutions:
- Compatible with Femtonics microscopes (SMART with tiltable or normal objective arm, Platform), and with an LN stage
- The condenser, condenser holder or the whole module is removable prior to in vivo measurements, owing to the quick release adapter placed on the fix mount to the optical table
MULTIPLE BEAM PATH
for uncaging and optogenetics
Technology Overview
The optomechanical design of the light path enables us to direct more beams into the microscope, utilizing the same light path. We offer secondary, fine-tuned laser sources for a wide range of biophotonics applications. See more: Optogenetics, Uncaging.
Features
- Additional IR or CW laser(s) coupled to the existing light path
- Lightpath can be optimized for three wavelength ranges (450-1100 or 700-1100 or 900-1300 nm)
- Full optical engineering
- Software controlled operation
Benefits
- Photostimulation with IR or visible light
- Flexible stimulation patterns supporting stimulation of selected regions
- Two-photon uncaging studies
- Visible light optogenetics:
- ChR2 activation with 473 nm laser
- NpHR activation with 561 nm laser
FEMTO3D Atlas
The FEMTO3D Atlas software:
- our measurement control and analysis software suite powering the FEMTO3D Atlas,
- supports a multitude of imaging workflows from fast planar imaging to patterned 3D photostimulation and simultaneous multicolor activity recording.
Find 3D scanning modes and other features on the Atlas Software product page.
FEMTOSmart
The FEMTOSmart software:
- powers our conventional 2D two-photon microscopes,
- offers streamlined simplicity for efficient, standardized data collection,
- offers all the essential functions for conventional experimental scenarios, including:
- fast planar and Z-stack recordings,
- scanning along 2D trajectories,
- photostimulation,
- a compact set of tools for data visualization and analysis,
- hardware controls arranged in an ergonomically designed interface.
Analysis, programming tool, automation
- Our microscopes can be extended by optional modules implementing specific measurement or analysis functions.
- Extending or automating available acquisition control and analysis functions is possible by interfacing custom scripts to Atlas or FEMTOSmart microscopes.
CO2 INCUBATOR*
to monitor CO2 pressure
Technology Overview
CO2 incubator is a device used to grow and nurture microbiological cultures or cell cultures. The incubator maintains optimal temperature, humidity, furthermore it evenly maintains and regulates the carbon dioxide content of the atmosphere inside. The device is essential for many kinds of research projects in various fields of science, such as cell biology, microbiology and molecular biology.
Features and benefits:
- 140℃ Dry Heat Sterilization
- In-chamber HEPA to filter microorganisms
- Interior containing copper to prevent the growth of undesirable bacteria
- Sensitive heat-resistant IR Sensor
- 7-inch touchscreen controller for real-time display
- Automatic data recording and curve display
- Fast heating system
- Eassily removable water tray provides high humidity
- Accurate IR sensor monitors CO2 changes
- Monitoring of CO2 pressure
*Special promotion item.
ANESTHESIA & STEREOTAXIC SOLUTION*
for rodents
Technology Overview
Changing the different types of stereotaxic instruments and adaptors enables you to perform a plathora of animal experiments, such as neurological disease experiments (Parkinson or Alzheimer’s disease, craniocerebral injury, spinal cord injury, etc.), brain drug injection, cannula implantation, physiological experiments (such as nerve stimulation, signal recording, wireless telemetry, etc.), microdialysis experiments (probe implantation and short-term dialysis) and many others.
The device is compatible with mouse and rat models.
ACTIVE ANESTHESIA GAS SCAVENGER*
for rodents
Technology Overview
The experimental system is equipped with a gas recovery system to provide a safe experimental environment and to maximize the protection of the operator.
Shape the product to your needs: replacing the different types of anesthesia devices and masks enables you to perform different types of animal experiments, such as creating cerebral ischemia and myocardial ischemia models, osteoporosis models, performing tail vein injection, abdominal aorta blood sampling, heart blood sampling, ventricular injection, tissue / organ removal and other types of surgeries for your experiments.
The device is compatible with mouse and rat models.
*Special promotion item.
LASER SPECKLE IMAGING SYSTEM*
Technology Overview
A blood perfusion imager based on the Laser Speckle Contrast Imaging (LSCI) technology. LSCI is a non-invasive optical imaging method used to obtain blood flow information in a wide rnage of applications, in ways that were not possible in the past. It allows visualization of tissue blood perfusion and imaging with high temporal (up to 140 fps) and spatial (2048×2048 pixels) resolution. The imager does not interfere with tissue perfusion, as no contact with the tissue is needed, and no dyes or tracer elements are neccessary.
Features and benefits:
- Online and offline analysis of vascular perfusion volume, tube diameter, tube angle and other data
- Multiple data acquisition methods including continuous acquisition, specified time interval acquisition, specified frame number acqusition, etc.
- Easy to use – indicating laser plus autofocus function
- High-definition and high-speed camera for images of superb quality
- For a wide variety of applications
*Special promotion item.
FIBER PHOTOMETRY SYSTEM*
Technology Overview
Aimed at detecting the activity change of neurons in the central nervous system in real-time, fiber photometry sums up the overall fluorescence of neurons to express their summed activity. Based on the reference channel of 410 nm, the RWD Fiber Photometry System adopts 470 nm and 560 nm dual-wavelength excitation, reducing background noise effectively. The significance of the reference channel lies in increasing self-control, reducing spontaneous fluorescence, eliminating signal artifacts caused by the animal’s intense movement and fiber winding, expressing two kinds of proteins at the same site, detecting levels of calcium change in different groups of neurons, and truly realizing the recording and analysis of dual-color fluorescence signals.
Features and benefits:
- Can support up to 9 channels, suitable for high-throughput calcium signal acquisition or simultaneous recording of multiple neural nucleus groups
- The software presets four LED excitation output modes, which can be applied to different experimental application scenarios
- Ultra-high sensitivity scientific research-grade CMOS camera, with higher quantum conversion efficiency, and the acquisition frequency can reach 300fps
- Integrated output port and pull-top cover design make the experimental fluorescence signal path less disturbed by the external environment and easy to operate
- Maximum capacity: 24 × 1.5/2.0 mL
- Maximum speed: 21,130 × g (15,000 rpm)
- Temperature control range: -10 °C to +40 °C, only takes 8 minutes to cool from room temperature to 4°C (Fast temperature mode); the temperature remains 4°C at the highest speed.
- RT mode and Cooling mode
- Automatic countdown mode
- Alarm system indicating over-speed and over-temperature
- Touch screen operation interface
- Rear-mounted compressor design
- Display: 4.3-inch touchscreen, 800*480 px
- Dimensions (W×D×H): 300mm*514mm*297mm
*Special promotion item.
IMPACTOR*
Technology Overview
Impactors are unique tools used for the induction of traumatic brain injury in animal models. They allow the experimenters to create a neurotrauma model with a high degree of reproducibility when it comes to the direction of impact and position of the injury. The pneumatically driven stereotaxic impactor is the most promising tool used for controlled cortical impact modeling: it delivers a precise and accurate injury to neocortex to mimic the significant physiological, histological, and behavioral aspects of traumatic brain injury and closed head injury. Choose from several user-friendly models, adaptable to various animal models with rodent or monkey adapters.
Features and benefits:
- New design & easy operation
- Precise control of speed, dwell time and strike depth,
- Thespinal cord adapters provide stable spinal fixation and improve the success rate of modeling
- Accurate tools with high efficiency, high repeatability, and stability
*Special promotion item.
VENTILATOR*
Technology Overview
Animal ventilators are the most common instruments for assisting animal respiration and are widely used by researchers, in preclinical and clinical medicine, in veterinary medicine, respiratory management during animal surgery and emergency treatment. This smart ventilator was designed for animal research applications and is widely using on subjects ranging in size from mice to guinea pigs. The ventilator provides to maintain versatility and ease of use while fostering safe and physiologically accurate mechanical ventilation conditions.
Features and benefits:
- Safe and effective intermittent positive pressure ventilation (IPPV), volume and pressure modes available
- Small size, compact layout, flexible operation
- 7 inches LCD touch screen, wide-angle visual
- Breathing frequency can be switched between 10 ~ 300bpm; tidal volume range: 0.05ml ~ 5ml
- Input power range: 100 ~ 240V, 50/60Hz, 24V, 40W, 1.67A adapter DC power supply, safe and effective
*Special promotion item.
AUTOMATED CELL COUNTER*
Technology Overview
The automated cell counter is a reliable cell counting devices with its highly intelligent analysis software and excellent microscopy optics structure. It can accurately identify the live cells, dead cells and the ones with specific fluorescence in a few seconds, which may liberate researchers from heavy work of daily cell counting.
Features and benefits:
- Accurate: cluster recognition algorithm, super clear imaging system, multi-application scenario incl. fluo
- Simple: 7-inch touch screen, auto focus & auto exposure, quick-count mode
- Reliable: automatic data record system, fl mode for complex sample analysis, suitable for different types of cellls
*Special promotion item.
AUTOMATED STEREOTAXIC INSTRUMENT*
for craniotomy and brain positioning
Technology Overview
An automatic and intelligent brain stereotaxic instrument, for craniotomy and precise positioning of brain regions in animal models. Compared to traditional manual positioners, the electric positioner can automatically calibrate and identify Bregma points, record and store multiple coordinate points, and display probe position in real time. With a resolution of 1μm.
Features and benefits:
- High-precision stepping motor drive
- Integrated mouse and rat brain maps in the software, can be displayed in 3D to assist in quick positioning
- Target position can be set in the software automatically
- The software interface can display probe trajectory and position in real time
- Multi-point program setting function, which can store and mark 10 coordinate points
- Can accurately control the depth of entry
- Two Optional control methods: computer or micro-operation control
- Set Bregma point with one click
- The software presets various tasks (automatic craniotomy, virus injection, etc.), for a high variety of experimental needs
- Displacement safety: prompt error alarm function
- Software operating system is compatible with Win7, Win10
*Special promotion item.
INTELLIGENT OPTOGENETICS SYSTEM*
for light-controlled neuromodulation
Technology Overview
The unmatched spatiotemporal precision and high tunability make optogenetics a powerful tool in neural research. This device integrates light source output and control into one tool, which is simpler and provides a more stable performance than other solutions. It comes with an intelligent design. Fields of application include research of neural circuits, learning and memory research, research of movement, addiction, sleep disorders, depression, anxiety, and Parkinson’s disease.
Features and benefits:
- All in one machine
- Touch screen operation interface
- Convenient parameter setting
- Control and visible waveform state
- Group custom editing and data import or export available
- Compatible with external behavior equipment, electrophysiology, calcium imaging equipment, etc.
*Special promotion item.
MINUX ROTARY MICROTOME*
for preparing tissue sections
Technology Overview
Tissue sectioning made intelligent, precise, easy, and safe: this device provides reproducible, thin, serial sections of high quality. Comes with a patented visual pointer for quick and accurate section positioning. With a section thickness set range of 0,5-100µm.
Features and benefits:
- Touch screen operation interface
- Side sampling knob for comfortable operation
- Knife guarding and retracting device for safe operation
- Adjustable speed (0-1800μm/s) for better sample control
- Alarm function and dual lock system for the handwheel
- The half-knife repair function simplifies the operation process, saves time and effort
- Sample position memory function, one-click reset to improve sectioning efficiency
- Record system to facilitate information tracking
*Special promotion item.
CRYOSTATS*
Features and benefits:
- The half-knife repair function simplifies the operation process.
- Automatic knife setting can save operation time and enables fast and accurate sectioning.
- Case and sample head dual cooling mode.
- Both the freezing table and the heat absorption block can quickly cool the sample. The heat absorption block flattens the sample and reduces tissue damage.
- Sample head adjustment: X / Y axis direction 8 °. Z axis can be rotated in 360°.
- Anti-roll plate to prevent tissue sections from curling.
- UV disinfection.
Technical parameters:
- Section Thickness Range: 0.50-100 μm
- Trimming Section Thickness Range: 1-600 μm
- Electric Sectioning Speed: 0-1800μm ajustable
- Specimen Retraction: 20±5 μm
- Specimen Feed: 28mm
- Vertical Stroke: 70 mm
- Specimen Orientation: X/Y axis 8°
- Number of cooling sites: 15
- Number of fast cooling sites: 2
- Refrigeration temperature: 0℃-35℃
- Sample head minimum cooling temperature: -50℃
*Special promotion item.
MICROCENTRIFUGES*
Features and benefits:
- Maximum capacity: 24 × 1.5/2.0 mL
- Maximum speed: 21,130 × g (15,000 rpm)
- Temperature control range: -10 °C to +40 °C, only takes 8 minutes to cool from room temperature to 4°C (Fast temperature mode); the temperature remains 4°C at the highest speed.
- RT mode and Cooling mode
- Automatic countdown mode
- Alarm system indicating over-speed and over-temperature
- Touch screen operation interface
- Rear-mounted compressor design
- Display: 4.3-inch touchscreen, 800*480 px
- Dimensions (W×D×H): 300mm*514mm*297mm
*Special promotion item.
HEAD HOLDERS
for rodents
Technology Overview
Head holder stands and head plates fix the rodent’s head in different positions, enabling precise measurements in the brain. Three types of head holders are available with different dimensions. For anesthetized rodents, we offer a heating pad coupled to a holder, or for behaving animals a stand that ensures access to jetballs, treadmills, or other devices. See more at Behavioral Studies.
Flexible head holder
Offering maximum flexible adjustment for positioning the head, this holder fixes the scull from one side. The head plate can be moved in three directions of the space and also along the bridge. It is offered for difficult to access parts such as eyes or auditory cortex, and anesthetized rodents.
Stabilized head holder
Stabilized head holder is attached to the rodent’s head at two sides. It is ideal for craniotomy or other surgery where the head of the animal model has to be fixed. It can be coupled to the Gramophone.
DNI-GLU-TFA
Technology Overview
This dinitro-indoline-masked form of glutamate releases the bioactive glutamate more rapidly than any other commercially available compound. It was developed for high-quantum yield requiring less irradiation for release, so its effective concentration is lower than other caging scaffolds. The caged compound exists as trifluoroacetic acid salted form (DNI-Glu*TFA) ensuring good solubility, stability and low hygroscopicity. DNI-Glu is a compound developed in-house, only available from Femtonics.
See more at Uncaging.
Features
- Photostimulation with two-photon laser
- High quantum-yield
- Effective at low concentration
- Seven-fold higher quantum yield than any other caged form
- Stable and soluble
- Primarily used for in vitro experiments
Benefits
- High excitatory postsynaptic potential and high Ca2+ transient as a response to the photo release
- Less illumination is sufficient to elicit the same response as with alternative compounds
- Elicits large transients or regenerative activity
- Applicable for receptor mapping experiments
References
Endocannabinoid Signaling Mediates Local Dendritic Coordination between Excitatory and Inhibitory Synapses.
Hai Yin Hu, Dennis L.H. Kruijssen, Cátia P. Frias, Balázs Rózsa, Casper C. Hoogenraad, Cell Reports (2019)
A compact holographic projector module for high-resolution 3D multi-site two-photon photostimulation.
Mary Ann Go, Max Mueller, Michael Lawrence Castañares, Veronica Egger, Vincent R. Daria, PLOS One (2019)
Coincidence Detection within the Excitable Rat Olfactory Bulb Granule Cell Spines.
S. Sara Aghvami, Max Müller, Babak N. Araabi and Veronica Egger, J. Neurosci. (2019)
Voltage Gated Calcium Channel Activation by Backpropagating Action Potentials Downregulates NMDAR Function
Anne-Kathrin Theis, Balázs Rózsa, Gergely Katona, Dietmar Schmitz and Friedrich W. Johenning, Front. Cell. Neurosci. (2018)
High efficiency two-photon uncaging coupled by the correction of spontaneous hydrolysis.
Dénes Pálfi, Balázs Chiovini, Gergely Szalay, Attila Kaszás, Gergely F. Turi, Gergely Katona, Péter Ábrányi-Balogh, Milán Szőri, Attila Potor, Orsolya Frigyesi, Csilla Lukácsné Haveland, Zoltán Szadai, Miklós Madarász, Anikó Vasanits-Zsigrai, Ibolya Molnár-Perl, Béla Viskolcz, Imre G. Csizmadia, Zoltán Mucsi and Balázs Rózsa, Org. Biomol. Chem. (2018)
Super-Resolution Imaging of the Extracellular Space in Living Brain Tissue
Jan Tønnesen, V.V.G. Krishna Inavalli, U. Valentin Nägerl, Cell (2018)
Imaging membrane potential changes from dendritic spines using computer-generated holography.
Dimitrii Tanese, Ju-Yun Weng, Valeria Zampini, Vincent de-Sars, Marco Canepari, Balazs J. Rozsa, Valentina Emiliani, Dejan Zecevic, Neurophotonics (2017)
Cell-type–specific inhibition of the dendritic plateau potential in striatal spiny projection neurons.
Kai Du, Yu-Wei Wu, Robert Lindroos, Yu Liu, Balázs Rózsa, Gergely Katona, Jun B. Ding, and Jeanette Hellgren Kotaleski, PNAS (2017)
Electrical behaviour of dendritic spines as revealed by voltage imaging.
Marko A. Popovic, Nicholas Carnevale, Balazs Rozsa; Dejan Zecevic, Nature Communications (2015)
Local Postsynaptic Voltage-Gated Sodium Channel Activation in Dendritic Spines of Olfactory Bulb Granule Cells.
Wolfgang G. Bywalez, Dinu Patirniche, Vanessa Rupprecht, Martin Stemmler, Andreas;V.M. Herz, Denes Palfi, Balazs Rozsa, Veronica Egger, Neuron (2015)
Quantitation of various indolinyl caged glutamates as their o-phthalaldehyde derivatives by high performance liquid chromatography coupled with tandem spectroscopic detections: derivatization, stoichiometry and stability studies.
Vasanits-Zsigrai A, Majercsik O, Toth G, Csampai A, Haveland-Lukacs C, Palfi D, Szadai Z, Rozsa B, Molnar-Perl I, J Chromatogr A. (2015)
Dendritic spikes induce ripples in parvalbumin interneurons during hippocampal sharp waves.
B Chiovini, G F Turi, G Katona, A Kaszas, D Palfi, P Maak, G Szalay, M F Szabo, Z Szadai, Sz Kali and B Rozsa, Neuron (2014)
Introduction
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Solution
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EXTREMELY LARG SPACE UNDER THE OBJECTIVE FEMTO SMART MICROSCOPES
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FEMTONICS GRAMOPHONE
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Femto3d
Atlas
All of our knowledge and results of acousto-optic technology have been poured into our new product, the Femto3D Atlas which has become the peak of the evolution of Femtonics acousto-optic microscopes. Atlas combines an intoxicating blend of high-tech science, engineering, refinement in 3D measurements. Using Atlas, researchers are capable of scanning neuronal, dendritic, and other neuropil activities in 3D about one million times faster compared to classical scanning methods with preserved two-photon resolution thanks to a unique acousto-optical scanner and the superior optical design. Combining its unique fast 3D imaging feature with the property that it implements and goes beyond the traditional galvo and resonant scanner-based microscope imaging functions, Atlas provides an all-in-one solution into the scientists’ hand.
FEATURES AND BENEFITS
- 3D imaging extension for any existing microscopes and individual system
- Simultaneous read out of neural activity on both the somatic and dendritic scales
- Increased measurement speed and signal-to-noise ratio by several orders of magnitude
- 3D Network imaging of over 2000 soma distributed in 3D
- Imaging of dendritic branches through layers
- Simultaneous imaging of over thousands of spines
- Unique, flexible scanning methods supporting in vivo and in vitro measurements
- In vivo 3D motion correction by specialized scanning patterns
- High-speed 3D random-access ROI scanning: scanning speed up to 30 kHz to any points in 3D
- High-speed raster scanning: 40 fps at 510x510, and even 3000 fps at reduced FOV
- Improved excitation efficiency for large 3D scanning volume with GECIs
- Automatic wavelength tuning between 750 - 1050 nm
- Functional imaging correlated with electrophysiological data
- Calcium imaging during behavior
- 3D random-access photo stimulation for optogenetics
Femto
Smart
The FemtoSmart series is the next step in classical two-photon microscopy at Femtonics, fully customizable two-photon microscopes. Their special feature is the elevated body which can move in X, Y, and Z directions, providing ample room under the objective for optimal positioning of your sample. This feature makes them suitable for model organisms ranging from zebra fish larvae, through mice navigating in virtual reality to even non-human primates. The microscope’s modular nature allows us to assemble the components, and recombine and upgrade the system to perfectly fit the customer’s needs. FemtoSmart product line contains galvo and/or resonant scanner-based systems which can be equipped with a lot of optional modules enabling it to be adapted to a wide range of biological applications, such as optogenetics, uncaging and dendritic imaging.
FEATURES AND BENEFITS
- Elevated, column-based body
- XYZ positioning
- Tilting objective
- High level of modularity
- Galvanometric, resonant, or both scanning possibilities
- Galvo Scanner, focusing on regions-of-interest
- Resonant Scanner, fast frame and volume scanning
- Dual Scanner, all advantages of Galvo and Resonant microscopes
- Travelling detector system
- For in vivo studies
- Field upgradability
- Patented imaging technologies
- Flexible scanning methods
- Maximal photon collection