FEMTO3D Atlas

FEMTO3D Atlas

Image more than 2,000 neurons in 3D, at 100 kilohertz speed, in freely behaving animals.

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Key features

Check 3D random-access scanning, up to 1,000,000x faster than classical methods

Check 3D network imaging of more than 2,000 neurons in a single session

Check Dendritic and spine imaging through cortical layers, without interruption

Check Voltage imaging at 100 kHz, fast enough to resolve single action potentials

Check Simultaneous 3D imaging and 3D photostimulation

Check Automatic wavelength tuning, from 750 to 1064 nm

Features

3D imaging at the speed of the brain

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Advanced 3D Imaging

Capture neural activity from large networks down to individual dendrites and arbitrary regions with flexible, high-speed 3D scanning.

In Vivo Imaging in Behaving Animals

Perform high-speed functional measurements during complex behavioral experiments.

Functional Measurements & 3D Photostimulation

Combine precise 3D optical stimulation with functional imaging to manipulate and observe neuronal circuits.

Standard Objective Unit
Standard Objective Unit

The Standard Objective Unit's green LED illumination provides high-contrast vascular visualization for precise in vivo navigation, patch-pipette positioning, and reliable relocation of chronic imaging sites.

Specifications

Explore the technical specifications behind precise, high-performance two-photon imaging

Imaging Capacity

More info

Scanning Performance

More info

Image Stability & Sensitivity

More info

Excitation & Functional Imaging

More info

System Integration

More info
Prof. Botond Roska
What you can do now is look simultaneously at all 150-200 presynaptic neurons that give input to your postsynaptic neuron and what they do at a given moment. You can really start to analyse how the brain computes in real time.

Prof. Botond Roska

Institute of Molecular and Clinical Opthalmology,
Basel Switzerland

Samples

See what researchers are imaging with FEMTO3D Atlas

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In vitro JEDI-2P measurement combined with uncaging

In vitro JEDI-2P measurement combined with uncaging

Lifetime trace, tilted: X: -5.9° Y: 4.1°

Lifetime trace, tilted: X: -5.9° Y: 4.1°

3D Chessboard Scanning in software

Interviews & Webinars

The methods, explained by the people who use them

webinar

Tune in to the brain - 3D acousto-optic technology in multiphoton microscopy

interview

An interview with Tristan Geiller: Shining a new light on memory formation

interview

Interview with neuroscience researcher, Christopher Makinson, PHD.

Downloads

Everything you need to evaluate the FEMTO3D Atlas

Voltage Imaging on a 2P Acousto-Optic (AO) System

whitepaper

Voltage Imaging on a 2P Acousto-Optic (AO) System
Photostimulation and Uncaging with Acousto-Optics

whitepaper

Photostimulation and Uncaging with Acousto-Optics
FEMTO3D Atlas Plug&Play

brochure

FEMTO3D Atlas Plug&Play
Discover Our Applications

brochure

Discover Our Applications
Prof. Dr. Jochen Herms
I am deeply impressed with your system. The training you provided was very effective — the microscope is working and stable, even without having you around.

Prof. Dr. Jochen Herms

DZNE, Munich

Modules

Extend the Atlas for your experiment

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FemtoStage

Optional modules

FemtoStage

The FemtoStage is a high-precision motorized XY stage with flexible, customizable sample handling for a wide range of in vivo and in vitro neuroscience experiments.

In-Vitro module

Optional modules

In-Vitro module

Flexible in vitro imaging for acute brain slices, cultured cells, and tissues combines gradient contrast illumination with efficient visible and infrared detection for reliable patch-clamping and high-SNR multimodal imaging.

Moculus

Optional modules

Moculus

The Mouse Virtual Reality system combines immersive, customizable environments with head-fixed high-resolution imaging to enable naturalistic behavior, precise stimulus control, and up to 300× faster visual learning.

4D Beam Conditioning Unit

Optional modules

4D Beam Conditioning Unit

The 4D-BCU suppresses beam dispersion, provides precise control over beam diameter, reduces beam divergence, and minimizes beam displacement.

FLIM Objective Unit

Optional modules

FLIM Objective Unit

High-performance Time-Correlated Single Photon Counting (TCSPC) and Fluorescence Lifetime Imaging (FLIM) provide sub-picosecond, concentration-independent fluorescence lifetime measurements for detailed analysis of molecular interactions and biophysical properties.

Epifluorescent Objective Unit

Optional modules

Epifluorescent Objective Unit

The Epifluorescence Objective Unit provides uniform, wavelength-selective fluorescence illumination and CMOS-based detection with flexible, software-controlled filter configurations for a wide range of fluorophores.

Related applications

Find the application closest to your research

Decorative
Events

Check out our upcoming events and workshops.

23

Nov

conference

BNMI 2026

Levi, Finnland

Péter Gál

International Sales Representative

Péter Gál

Tamás Tompa PhD

Application Specialist

Tamás Tompa PhD

Levi, Finnland

View Details

14

Nov

conference

Society for Neuroscience 2026, SfN

Washington DC, USA

Péter Gál

International Sales Representative

Péter Gál

Éva Árgyelán

Head of Sales Department

Éva Árgyelán

Patrick Stemkowski PhD

North American Sales Representative

Patrick Stemkowski PhD

Viktória Kiss PhD

Head of Application Specialist Department

Viktória Kiss PhD

Dénes Pálfi PhD

Application Specialist

Dénes Pálfi PhD

Washington DC, USA

View Details
Every FEMTO3D Atlas ships with the team behind it
Service & support

Every FEMTO3D Atlas ships with the team behind it

Installation, training, and ongoing support are handled directly by Femtonics engineers, not a distributor. From first light to advanced experiments, the people who built the system are the ones helping you run it.

Control the FEMTO3D Atlas the way you actually work
Software

Control the FEMTO3D Atlas the way you actually work

Acquisition, scanning parameters, and real-time monitoring in one interface. Set your targets, run the experiment, and move straight into analysis without switching tools.