Highlighted publications
Theoretical Design, Synthesis, and In Vitro Neurobiological Applications of a Highly Efficient Two-Photon Caged GABA Validated on an Epileptic Case
03rd June 2021
B. Chiovini, D. Pálfi, M. Majoros, G. Juhász, G. Szalay, G. Katona, M. Szőri, O. Frigyesi, Cs. Lukácsné Haveland, G. Szabó, F. Erdélyi, Z. Máté, Z. Szadai, M. Madarász, M. Dékány, I. G. Csizmadia, E. Kovács, B. Rózsa, and Z. Mucsi
Two-photon GCaMP6f imaging of infrared neural stimulation evoked calcium signals in mouse cortical neurons in vivo
07th May 2021
A. Kaszas, G. Szalay, A. Slézia, A. Bojdán, I. Vanzetta, B. Hangya, B. Rózsa, R. O’Connor & D. Moreau
Large-Scale 3D Two-Photon Imaging of Molecularly Identified CA1 Interneuron Dynamics in Behaving Mice
05th October 2020
T. Geiller, B. Vancura, S. Terada, E. Troullinou, S. Chavlis, G. Tsagkatakis, P. Tsakalides, K. Ócsai, P. Poirazi, B. J. Rózsa, A. Losonczy
Cell Types of the Human Retina and Its Organoids at Single-Cell Resolution
05th October 2020
C. S. Cowan, M. Renner, M. De Gennaro, B. Gross-Scherf, D.Goldblum, Y. Hou, M. Munz, T. M. Rodrigues, J. Krol, T. Szikra, R. Cuttat, A. Waldt, P. Papasaikas, R. Diggelmann, C. P. Patino-Alvarez, P. Galliker, S. E. Spirig, D. Pavlinic, N. Gerber-Hollbach, S. Schuierer, A. Srdanovic, M. Balogh, R. Panero, A. Kusnyerik, A. Szabo, M. B. Stadler, S. Orgül, S. Picelli, P. W. Hasler, A. Hierlemann, H. P. N. Scholl, G. Roma, F. Nigsch, B. Roska
Restoring light sensitivity using tunable near-infrared sensors
05th June 2020
D. Nelidova, R. K. Morikawa, C. S. Cowan, Z. Raics, D. Goldblum, H. P. N. Scholl, T. Szikra, A. Szabo, D. Hillier, B. Roska
Dendritic action potentials and computation in human layer 2/3 cortical neurons
03rd January 2020
A. Gidon, T. A. Zolnik, P. Fidzinski, F. Bolduan, A. Papoutsi, P. Poirazi, M. Holtkamp, I. Vida, M. E. Larkum
High and asymmetric somato-dendritic coupling of V1 layer 5 neurons independent of visual stimulation and locomotion
27th December 2019
V. Francioni, Z. Padamsey, N. L Rochefort
Microglia monitor and protect neuronal function through specialized somatic purinergic junctions
31st January 2020
Cs. Cserép, B. Pósfai, N. Lénárt, R. Fekete, Zs. I. László, Zs. Lele, B. Orsolits, G. Molnár, S. Heindl, A. D. Schwarcz, K. Ujvári, Zs. Környei, K. Tóth, E. Szabadits, B. Sperlágh, M. Baranyi, L. Csiba, T. Hortobágyi, Zs. Maglóczky, B. Martinecz, G. Szabó, F. Erdélyi, R. Szipőcs, M. M. Tamkun, B. Gesierich, M. Duering, I. Katona, A. Liesz, G. Tamás, Á. Dénes
Microglia protect against brain injury and their selective elimination dysregulates neuronal network activity after stroke
03rd May 2016
G. Szalay, B. Martinecz, N. Lénárt, Zs. Környei, B. Orsolits, L. Judák, E. Császár, R. Fekete, B. L. West, G. Katona, B. Rózsa & Á. Dénes
Fast 3D Imaging of Spine, Dendritic, and Neuronal Assemblies in Behaving Animals
01st August 2016
G. Szalay, L. Judak, G. Katona, K. Ocsai, G. Juhasz, M. Veress, Z. Szadai, A. Feher, T. Tompa, B. Chiovini, P. Maak, B. Rozsa
Electrical behaviour of dendritic spines as revealed by voltage imaging
05th October 2015
M. A. Popovic, N. Carnevale, B. Rozsa & D. Zecevic
Time-Resolved Imaging Reveals Heterogeneous Landscapes of Nanomolar Ca2+ in Neurons and Astroglia
21st October 2015
Single-cell–initiated monosynaptic tracing reveals layer-specific cortical network modules
03rd July 2015
Combined two-photon imaging, electrophysiological, and anatomical investigation of the human neocortex in vitro
11th September 2014
B. P. Kerekes, K. Toth, A. Kaszas, B. Chiovini, Z. Szadai, G. Szalay, D. Palfi, A. Bago, K. Spitzer, B. Rozsa, I. Ulbert, L. Wittner
Dendritic Spikes Induce Ripples in Parvalbumin Interneurons during Hippocampal Sharp Waves
21st May 2014
Fast two-photon in vivo imaging with three-dimensional random-access scanning in large tissue volumes
08th January 2012
G. Katona, G. Szalay, P. Maák, A. Kaszás, M. Veress, D. Hillier, B. Chiovini, E. S. Vizi, B. Roska & B. Rózsa
Large-Scale 3D Two-Photon Imaging of Molecularly Identified CA1 Interneuron Dynamics in Behaving Mice
Tristan Geiller, Bert Vancura, Satoshi Terada, Eirini Troullinou, Spyridon Chavlis, Grigorios Tsagkatakis, Panagiotis Tsakalides, Katalin Ócsai, Panayiota Poirazi, Balázs J. Rózsa, Attila Losonczy
05th October 2020
A recently published breakthrough research can shine a light on the previously unknown in vivo activity dynamics of interneurons during behavior.
Interneurons, the inhibitory cells crucial for keeping brain activity in balance, have been notoriously difficult to study. However, Geiller et al. from the team of Attila Losonczy (Professor of Neuroscience at Columbia University) have followed a large network of these sparsely distributed cells in the brain of behaving mice in real-time in 3D, identifying interneuron types and their activity dynamics simultaneously. In their study published in Neuron, they measured calcium activity from nearly 250 interneurons across all hippocampal layers in the CA1 area in an 800 × 800 × 500 µm volume simultaneously with the chessboard scanning mode of the FEMTO3D Atlas, while the mouse was performing behavioral tasks on a treadmill. The molecular identity of imaged interneurons was retrospectively determined by post hoc immunohistochemistry and they were carefully matched with the cells recorded by the Atlas. This is a great novelty, as, although the anatomical and molecular properties of interneurons from this area have been characterized, little is known about their population-level in vivo activity dynamics during behavior. This partly stems from the limited ability of traditional recording techniques (e.g. electrophysiology, one-photon, or conventional two-photon imaging). Ultimately, these findings can bring us closer to understanding the exact roles of these inhibitory neurons in the hippocampus by allowing us to study their activities and interactions in a wholly new way.
Image: Illustration of the research including examples of different types of hippocampal interneurons and their activity patterns published in the publication in Neuron (Credit: Losonczy lab)
In our newly released interview Attila Losonczy, MD, PhD, principal investigator at Columbia’s Mortimer B. Zuckerman Mind Brain Behavior Institute shares first-hand information about what kind of instruments and methods they used to investigate the brain’s ability to acquire and store information. Watch the interview here.
