FEMTOSmart software packs a range of features indispensable for efficient data acquisition, visualization and analysis. The clean, ergonomic user interface has been condensed from the experience of many scientists who have been using the FEMTOSmart microscopy platform.
Flexible and efficient data management
Resonant scanners acquire multichannel full field of view images at 30 frames per second (fps) and can even reach 1000 fps while recording from a smaller region. FEMTOSmart software handles the high data rates produced during acquisition with an optimized data handling architecture that can stream data to disk in real-time. Therefore, only storage size will limit the length of the experiment. The optimized data pipeline of the FEMTOSmart software features:
Simultaneous activity imaging and photostimulation
The FEMTOSmart software supports truly simultaneous multichannel imaging and photostimulation on hardware configurations equipped with two light paths (FEMTOSmart Dual). Cellular and subcellular structures can be photostimulated at user-positioned points, line segments or spirals. Light can be precisely dosed via the experiment protocol editor in which the user can quickly set up waveforms with millisecond precision to control light-path elements.
Region of interest visualization and analysis
Regions of interest (ROIs) can be selected in order to perform select real-time calculations simultaneously with image acquisition. ROIs may be chosen in various shapes and, for photoactivation, can be grouped together as separate populations. Possible ROI calculations include:
Application Programming Interface (FemtoAPI)
The application programming interface for our FEMTOSmart microscopy platform (FemtoAPI) lets you control your Femtonics microscope with MATLAB and Python scripts. Via FemtoAPI, one can create custom measurement protocols and integrate Femtonics microscopes to other lab software environments.
FemtoAPI based extensions of FEMTOSmart microscopes allow
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The FEMTOSmart software (MESC 4.0) for FEMTOSmart 2D systems.
Minimum system requirements