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OVERVIEW

The Tilting objective module provides a higher level of freedom in moving the objective at precise angles, thereby it is ideally useful for in vivo experiments, intravital imaging, deep brain imaging in rodents or even non-human primates.

The unit is driven by two motors which rotate the objective around horizontal and vertical axes. The movement is controlled by the microscope software and by the handwheel unit. The module also includes a Piezo objective positioner, ensuring additional movement of the objective in the Z direction for the proper orientation.

SPECIFICATION

  • rotation around horizontal axis: +90/-90º
  • rotation around vertical axis: +50/-50º
  • speed of rotation: 4º/sec
  • unidirectional repeatability < 0.02 mrad
  • bidirectional repeatability < 1 mrad
  • transmission over 80% (700-1100 or 900-1300 nm)
  • additional Z movement with piezo objective positioner: 400 µm
  • accepts both conventional and large back aperture objectives​
  • maximum number of mounted detectors: 3
  • can be equipped to all members of FemtoS and Femto2D series

BENEFITS

  • motorized, software controlled
  • fast z-stack acquisition also from tilted position
  • flexible objective positioning with high precision
  • backlash-free, high stability at all positions
  • minimal dispersion, no optical aberration
  • minimized optical light path, detectors equipped to tilting unit
  • optional green lamp illumination

APPLICATIONS

Novel surgical methods utilizing grin lenses or miniature prisms allow optical investigation of deep structures within the brain. Implementing these technologies is greatly enhanced by our motorized tilting objective:
  • aligning optical axis of the embedded optical elements is facilitated by the handwheel opetated multi-axis positioning of the grin lens/Smart microscope,
  • store and reuse optimal angles and positions when experimenting multiple times with the same animal,
  • utilize the units high-precision movements to fine adjust position and alignment without actually touching the assembly.