FemtoS-Resonant equipped with LED light source moduleAn enlarged surface or the entire FOV can be stimulated with LED light source. LEDs are available with different wavelengths exciting ChR2 at 473 nm or NpHR at 561 nm. The impulses of the light are precisely timed and highly repeatable fitting to optogenetics studies. We offer FemtoS-Resonant microscope to follow the changes on the whole field of view with high speed. FemtoS-Resonant can be equipped with CW and IR laser too for photostimulation. The LED light source is available to Femto2D-Resonant too.
For full field illumination
FemtoS-Galvo equipped with Multiple beam moduleTo stimulate cells or subcellular components selectively, the best solution is to steer the laser beam rapidly through optimised scanning patterns, such as point, line, spiral, zigzag, etc. by FemtoS-Galvo. Beside of the scanning along points and lines, which allows stimulation on in precise locations on spines or dendrites, the spiral and zigzag patterns covering larger region enable enough molecules to stimulate on the soma. As Multiple beam module, we offer continuous laser tuned 473 or 561 nm for ChR2/NpHR activation. Photostimulation with LED light source and precise two-photon activation of these molecules is also a viable option with FemtoS-Galvo. The multiple beam module is available to Femto2D-Galvo too.
Stimulation along ROIs
FemtoS-DualFemtoS-Dual microscope contains a galvo and resonant scanners in tandem combined the advantages of galvo and resonant microscopes. It offers possibility to simultaneous photostimulation and high speed imaging during optogenetics studies.
Simultaneous photoactivation and imaging
Holographic stimulationThe holographic stimulation is an extremely flexible method which produces simultaneous illumination in variable shape and size of multiple regions in the three-dimensional sample. Our Holographic module can be equipped to Femto2D-Galvo.
Stimulation in 3D
The simultaneous illumination is established by diffractive Spatial Light Modulator (SLM). The SLM has a parallel-aligned nematic liquid crystal layer in which the crystals disperse and shape the incoming laser beam and change the phase of light. This converted holographic beam excites multiple spots with arbitrary shapes in pre-determined depths simultaneously. The holographic unit is controlled by our MES software, which calculates the corresponding phase-hologram and addresses the pattern to the crystal layer.
- 3D pattern activation in a 350 x 400 x 400 µm3 stimulating volume (Olympus 20x, NA1)
- Simultaneous excitation in several depths with many ROIs (~20-30 cells in 3-5 planes)
- ROIs are defined as polygons with arbitrary shape and size
- Lateral resolution less than 3 µm, axial resolution less than 10 µm
- Activation time down to 10 µs or 5 ms depending on the shutter device
- Repetition rate of SLM: 60 Hz
- Large illumination region allows activation of sufficient number of photoactive molecules
- Wide laser wavelength range from the visible to the infrared regime
- Illumination does not depend on the scanning parameters
- Holographic system does not contain mechanically moving parts
Linking Neurons to Network Function and Behavior by Two-Photon Holographic Optogenetics and Volumetric Imaging. Dal Maschio M, Donovan JC, Helmbrecht TO, Baier H, Neuron (2017)
An optogenetic toolbox for unbiased discovery of functionally connected cells in neural circuits. Dominique Förster, Marco Dal Maschio, Eva Laurell, and Herwig Baier, Nature Communication (2017)