Technology Overview
This dinitro-indoline-masked form of glutamate releases the bioactive glutamate more rapidly than any other commercially available compound. It was developed for high-quantum yield requiring less irradiation for release, so its effective concentration is lower than other caging scaffolds. The caged compound exists as trifluoroacetic acid salted form (DNI-Glu*TFA) ensuring good solubility, stability and low hygroscopicity. DNI-Glu is a compound developed in-house, only available from Femtonics.
Features
- Photo stimulation with two-photon laser
- High quantum-yield
- Effective at low concentration
- Seven-fold higher quantum yield than any other caged form
- Stable and soluble
- Primarily used for in vitro experiments
Benefits
- High excitatory postsynaptic potential and high Ca2+ transient as a response to the photo release
- Less illumination is sufficient to elicit the same response as with alternative compounds
- Elicits large transients or regenerative activity
- Applicable for receptor mapping experiments
