FEMTO3D Atlas Plug & Play

ROLLS OUT OF THE BOX
Compact and arrives ready to operate

FUNCTIONAL REAL-TIME 3D IMAGING
Calcium imaging, voltage imaging

DEEP PENETRATION
Low phototoxicity, high optical quality

UNIQUE FLEXIBLE IMAGING METHODS
Supporting neurobiological applications

NETWORK IMAGING
Of over 2000 soma distributed in 3D

DENDRITIC IMAGING
And spine mapping without interruption

DURING BEHAVIOR
Real-time 3D motion correction

VOLTAGE IMAGING

The rapidly evolving voltage sensor technology determines the next decade of bio-imaging: they have faster kinetics than calcium sensors and can provide superior temporal resolution, capable of detecting spikes at frequencies greater than 100 Hz. Combined with the high sampling rate of the Atlas Plug & Play (up to 100kHz), they are suited for detecting ultrafast transients, such as action potentials (APs). The AO technology also surmounts mechanical distortion and jumping delay between ROIs, spending recording time only at structures of interest. The Atlas Plug & Play powerd by the AO technology, with real-time motion correction, is currently the only imaging device which can keep up with the speed of
firing neurons.

REAL-TIME 3D MOTION CORRECTION

Drawing reliable conclusions from data collected from behaving animals can be challenging, as artifacts arising from tissue movements can be difficult to discern from real activity signals. The Femtonics FocusPinner real-time 3D motion correction feature has been developed to eliminate motion artifacts during data acquisition not just along the axes of the imaged plane but also along the optical axis. By combining it with the scanning modes of the Atlas Plug & Play, neuronal activity data can be aquired without motion artifacts while the animal is performing tasks in virtual reality. FocusPinner will allow you to profit from unprecedented signal-to-noise ratio and save time by avoiding post-hoc processing.

ABOUT THE FEMTO3D Atlas Plug & Play

• In vivo functional imaging down to below 850 µm depth
• 500 µm × 500 µm × 650 µm scanning volume in vivo (with a 20x, NA=1.0 obj.)
(In case of excellent labelling it can be exteded to 800 µm x 800 µm x 1050 µm while keeping the good central resolution)
• Wavelength between 920 – 1040 nm
• Integrated automatic beam stabilization
• Integrated dispersion compensation unit for most effective excitation
• Diffraction limited, submicrometer resolution in the center (<450 nm)
• Scanning speed up to 100 kHz to any points in 3D
• 3D SCANNING MODES: random-access point, trajectory, tilted frame, volume, ribbon, snake, chessboard, multi-cube scanning
• Quasi-simultaneous 3D imaging and 3D photostimulation
• Real-time 3D Motion Correction to eliminate motion artifacts arising from tissue movements