• FLIM extension

    for fluorescence Lifetime Imaging

Technology Overview

Time correlated single photon counting and the derived fluorescence lifetime imaging measures the time delay between each emitted photon and the laser pulse eliciting it. This time is not affected by fluorophore concentration and excitation intensity fluctuations, however provides intimate information about molecular interactions and dynamics.

Related Publications:
Monitoring intracellular nanomolar calcium using fluorescence lifetime imaging.
Kaiyu Zheng, Thomas P Jensen & Dmitri A Rusakov, Nature Protocols (2018)
Monitoring single-synapse glutamate release and presynaptic calcium concentration in organised brain tissue.
Jensen TP, Zheng K, Tyurikova O, Reynolds JP, Rusakov DA, Cell Calcium (2017)
Time-Resolved Imaging Reveals Heterogeneous Landscapes of Nanomolar Ca2+ in Neurons and Astroglia
Kaiyu Zheng, Lucie Bard, James P. Reynolds, Claire King, Thomas P. Jensen, Alexander V. Gourine, Dmitri A. Rusakov, Neuron (2015)


  • high efficiency hybrid GaAsP PMTs for photon counting

  • no afterpulsing

  • multiple channels for two separated wavelengths

  • non-descanned design for high photon collection rate

  • minimized optical path length by travelling detector system

  • sub-picosecond temporal resolution

  • parallel conventional integrating and photon counting modes


  • absolute ion concentration measurement

  • characterization of bio-physical properties

Optional modules

Femtonics Technology