Breakthrough innovation in Multiphoton Microscopy

All of our knowledge and progress in the development of acousto-optic technology have been poured into our new product, the Femto3D Atlas, which has become the pinnacle of the evolution of Femtonics acousto-optic (AO) microscopes. Atlas combines an intoxicating blend of high-tech science, engineering and, refinement in 3D measurements. Using Atlas, researchers are capable of scanning neuronal, dendritic, and other neuropil activities in 3D about one million times faster compared to classical scanning methods with a preserved two-photon resolution.

The advantage of Femto3D Atlas

The advantage of Femto3D Atlas is the combination of flexibility and high-speed imaging: thanks to the fast XYZ scanning, scanning areas can be positioned in 3D arbitrarily, without mechanical restrictions, (1) data can be collected near simultaneously with 30 kHz from selected ROIs, and (2) the speed of frame scanning goes beyond the imaging rate of resonant microscopes, it reaches 40 fps on 510 x 510 pixels, 500 x 500 µm2. Combining its unique fast 3D imaging feature with the property that it implements and goes beyond the traditional galvo and resonant scanner-based microscope imaging functions, Atlas provides an all-in-one solution for researchers.

  • 3D imaging extension for any existing microscope and individual system
  • Simultaneous read out of neural activity on both the somatic and dendritic scales
  • Increased measurement speed and signal-to-noise ratio by several orders of magnitude
  • 3D Network imaging of over 2000 soma distributed in 3D
  • Imaging of dendritic branches through layers
  • Simultaneous imaging of over thousands of spines
  • Unique, flexible scanning methods supporting in vivo and in vitro measurements
  • In vivo 3D motion correction by specialized scanning patterns

  • High-speed 3D random-access ROI scanning: scanning speed up to 30 kHz to any points in 3D
  • High-speed raster scanning: 40 fps at 510×510, and even 3000 fps at reduced FOV
  • Improved excitation efficiency for large 3D scanning volumes with GECIs
  • Automatic wavelength tuning between 750 – 1050 nm
  • Functional imaging correlated with electrophysiological data
  • Calcium imaging during behavior
  • 3D random-access photostimulation for optogenetics

The system

The precisely designed frame provides an exceptional appearance and high stability for the Femto3D Atlas, enabling it to be coupled with numerous kinds of host microscopes. The Femto3D Atlas contains not only the AODs but the requiring light path with optics, control electronics and a work station, and it involves the full optical engineering, and our measurement control and analysis software. This system coupled with a host microscope, provides the above mentioned superior in vivo imaging parameters. For system compatibility and more details, please contact us.

The host microscope

The host microscope must have the following, essential properties: upright arrangement, upright camera output and a laser source. The Femto3D Atlas connects from an upper position to the host microscope through the camera port. In this arrangement, the camera port could be replaced. If the existing laser source is not equipped with a beam stabilization unit, our 4D beam condition unit ensures the precise operation of the acousto-optic scanner-based imaging. If there is no host microscope, we offer our basic, low-cost Femtonics 2P Platform system, which has been developed exclusively for the Femto3D Atlas.

Build your future with us

The Femto3D Atlas operating together with your microscope, provides an ultimate 3D imaging solution.

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